Carba NP法用于产碳青霉烯酶菌株快速检测的研究  

A study of Carba NP method in rapid detection of carbapenemase-producing strains

作　　者：袁敏[1] 龚林[1] 陈霞[1] 刘佳[1] 禹惠兰[1] 徐建国[1] 李娟[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所细菌耐药室/传染病预防控制国家重点实验室,北京102206

出　　处：《国际检验医学杂志》2013年第23期3105-3106,3109,共3页International Journal of Laboratory Medicine

基　　金：传染病预防控制国家重点实验室面上项目(2012SKLID2005)

摘　　要：目的探讨将Carba NP法用于产碳青酶烯酶肠杆菌科及假单胞菌属细菌的检测。方法将8株产碳青霉烯酶菌株作为研究对象,采用Carba NP法进行碳青霉烯酶检测,并将其与改良Hodge试验比较。结果 Carba NP法能够特异、灵敏地检测产碳青霉烯酶菌株。Carba NP试验Ⅰ能检测菌株是否产碳青霉烯酶,Carba NP试验Ⅱ能进一步判定产碳青酶烯酶菌株的组别。其检测方法较改良的Hodge试验更简单、快速。结论 Carba NP法操作快速、简单,有助于提高产碳青酶烯酶菌株的检出率及医院内感染的控制。Objective To explore the application of Carba NP method for detecting carbapenemase-producing Enterobacteriaceae and Pseudomonads.Methods Eight carbapenemase-producing strains were served as objects,Carba NP method was conduct to detect carbapenemase production and was compared with modified Hodge test.Results Carba NP method could detect carbapenemase-producing strains specifically and sensitively.Carba NPⅠcould identified whether the strains were carbapenemase producers and Carba NPⅡcould further discriminate the type of carbapenemase-producing strains.It was more easily and quickly.than modified Hodge test.Conclusion Carba NP method is rapid,simple and contribute to improve the detection rate of carbapenemase-producing strains and control the nosocomial infection.

关 键 词：肠杆菌属 假单胞菌属 碳青霉烯酶 

分 类 号：R440[医药卫生—诊断学]