游离脂肪酸对HepG2细胞及HPA-v成熟脂肪细胞miRNA-143表达的影响  

Effects of free fatty acid on miRNA-143 expression in HepG2 cells and HPA-v mature adipocytes

作　　者：王秀芳[1,2] 张向君[3] 徐广峰[2] 张乃键[1,2] 赵超[2] 赵亚萍[1,2] 

机构地区：[1]蚌埠医学院医学检验系,安徽蚌埠233030 [2]中国人民解放军第八二医院检验科,江苏淮安223001 [3]蚌埠医学院第一附属医院感控科,安徽蚌埠233030

出　　处：《国际检验医学杂志》2013年第23期3110-3111,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨游离脂肪酸(FFA)对人肝癌HepG2细胞及HPA-v成熟脂肪细胞miRNA-143表达的影响。方法体外培养HepG2细胞及人前体脂肪细胞HPA-v,将HPA-v前体脂肪细胞诱导分化为成熟脂肪细胞。用1mmol/L FFA分别干预HepG2细胞及HPA-v成熟脂肪细胞0、4、12、24h,采用实时定量逆转录-聚合酶链反应(qRT-PCR)技术检测细胞miRNA-143的表达水平。结果随FFA干预时间的延长,HepG2细胞miRNA-143表达增高(P<0.05),HPA-v成熟脂肪细胞中miRNA-143的表达降低(P<0.05)。结论 FFA对HepG2细胞及HPA-v成熟脂肪细胞miRNA-143表达具有不同的调节作用。Objective To explore the effects of free fatty acid（FFA）on miRNA-143 expression in human hepatoma HepG2 cells and HPA-v mature adipocytes.Methods HepG2 cells and human HPA-v preadipocytes were cultured in vitro.HPA-v preadipo- cytes were induced to differentiate into mature adipocytes.HepG2 cells and HPA-v mature adipocytes were treated with 1 mmol/L FFA for 0,4,12,24 h,respectively.Quantitative reverse transcriptase-polymerase chain reaction（qRT-PCR）technique was used to detect cellular miRNA-143 expression levels.Results miRNA-143 expression increased in HepG2 cells （P〈0.05） and decreased in HPA-v mature adipocytes （P〈0.05） with extension of FFA treatment time.Conclusion FFA shows different regulative roles on miRNA-143 expression in HepG2 cells and HPA-v mature adipocytes.

关 键 词：脂肪酸类 脂细胞 HEPG2 miRNA-143 

分 类 号：R589.2[医药卫生—内分泌]