慢性乙型肝炎患者血清补体C3b水平的研究  

A study on serum complement C3b levels of patients with chronic hepatitis B

作　　者：蒋静[1] 刘斌[2] 热依汗.谢以提 孟存仁[1] 

机构地区：[1]新疆医科大学第一附属医院检验科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院输血科,新疆乌鲁木齐830054

出　　处：《国际检验医学杂志》2013年第23期3116-3118,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(30660718);新疆维吾尔自治区病原生物学重点学科资助项目(BYX11001)

摘　　要：目的探讨慢性乙型肝炎患者血清补体C3b水平及其临床意义。方法选择乙型肝炎病毒(HBV)感染的慢性乙型肝炎患者140例,据患者血清中HBV e抗原(HBeAg)和丙氨酸氨基转氨酶(ALT)水平,将其分为6组:A组(HBeAg阳性,ALT正常)、B组(HBeAg阴性,ALT正常)、C组(HBeAg阳性,ALT轻、中度升高)、D组(HBeAg阳性,ALT高度升高)、E组(HBeAg阴性,ALT轻、中度升高)、F组(HBeAg阴性,ALT高度或重度升高)。另将20例健康者作为对照组。HBeAg采用酶联免疫吸附测定(ELISA)法,HBV-DNA采用荧光定量聚合酶链反应(PCR)法,ALT采用速率法,补体C3b采用ELISA-生物素亲和素抗体夹心法测定。结果 D组与F组患者血清C3b、ALT、天冬氨酸氨基转氨酶(AST)水平显著高于A、B、C、E组(P<0.05);而D组与F组比较,患者血清C3b、AST水平均无统计学差异(P>0.05)。对照组受检者血清C3b水平显著低于A、B、C、D、E、F组(P<0.05)。C3b水平与ALT、AST、HBsAg存在正相关(P<0.05),而与HBeAg和HBV-DNA无显著相关(P>0.05)。结论补体C3b水平与HBV的感染及肝细胞损伤程度有关,而与病毒复制的活跃程度无关,可作为感染和预后的辅助检测指标。Objective To investigate serum complement C3b levels of patients with chronic hepatitis B and its clinical signifi- cance.Methods 140 hepatitis B virus（HBV）-infected patients with chronic hepatitis B were enrolled and divided into 6 groups ac- cording to their serum levels of HBV e antigen（HBeAg） and alanine aminotransferase（ALT）：group A（HBeAg：positive,ALT： normal）,group B（HBeAg：negative,ALT：normal）,group C（HBeAg：positive,ALT：mildly or moderately elevated）,group D（HBeAg：positive,ALT：highly elevated）,group E（HBeAg：negative,ALT：mildly or moderately elevated）,group F（HBeAg： negative,ALT：highly or severely elevated）.Another 20 healthy people were served as the control group.Enzyme-linked immu- nosorbent assay （ELISA） was employed to detect HBeAg,fluorescence quantitative polymerase chain reaction（PCR）was used to measure HBV-DNA,rate method was adopted to detect ALT and biotin/avidin double-antibody sandwich ELISA was used to meas- ure complement C3b.Results Serum C3b,ALT,aspartate aminotransferase（AST）levels of patients in group D and group F were significantly higher than those in group A,B,C,E（P〈0.05）.Compared group D with group F,serum C3b,AST levels were not statistically different（P〈0.05）.Serum C3b levels of healthy people in control group were markedly lower than those in group A, B,C,D,E,F（P〈0.05）.C3b levels was positively correlated to ALT,AST and HBsAg levels（P〈0.05） and showed no significant correlation with HBeAg and HBV-DNA levels（P〈0.05）.Conclusion Complement C3b is related to degrees of HBV infection and hepatocyte damage rather than activity of viral replication,and can be used as an auxiliary detection indicator of HBV infection and prognosis.

关 键 词：补体C3b 肝炎病毒 乙型 丙氨酸氨基转氨酶类 天冬氨酸氨基转移酶类 

分 类 号：R512.62[医药卫生—内科学]