结核感染者外周血CD4^+T淋巴细胞亚群的检测及其意义  

Detection of CD4^+T lymphocyte subsets in peripheral blood of people infected with tuberculosis and its significance

作　　者：刘才冬[1] 张瑞生[1] 高应东[1] 金玲[1] 万芳[1] 夏永祥[1] 

机构地区：[1]南京医科大学附属南京医院/南京市第一医院检验科,江苏南京210006

出　　处：《国际检验医学杂志》2013年第23期3174-3175,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨结核感染者外周血CD4+T淋巴细胞亚群的检测及其临床意义。方法采用结核感染T淋巴细胞斑点试验(T-SPOT.TB)技术筛选结核患者与非结核患者各40例。采用流式细胞术检测其体内T淋巴细胞亚群,即调节性T淋巴细胞(Treg)与辅助性T淋巴细胞(TH)。结果结核组患者Treg细胞百分比明显高于对照组,TH1细胞百分比及TH1/TH2比值明显低于对照组(P<0.05)。两组TH1细胞百分比的差异无统计学意义(P>0.05)。结论结核感染者外周血Treg细胞数量上升,TH1细胞数量及TH1/TH2比例下降,机体免疫力的下降可能是结核感染的重要原因之一。Objective To explore the detection of CD4＋T lymphocyte subsets in peripheral blood of people infected with tuber- culosis（TB）and its significance.Methods TB-infected T lymphocyte spot test（T-SPOT.TB）was employed to screen for patients with TB and with non-TB,each of 40 cases.Flow cytometry was adopted to detect their T lymphocyte subsets,regulatory T lym- phocyte（Treg）and helper T lymphocytes（TH）in peripheral blood.Results The percentage of Treg cells of patients in TB group was significantly higher than that in control group.The percentage of TH1 cells and the ratio of TH1/TH2 were markedly lower than those in control group（P〈0.05）.The percentage of TH1 cells in the two groups showed no statistical significance（P〈0.05）. Conclusion The amount of Treg cells in peripheral blood of people infected with TB increases,while the amount of their TH1 cells and TH1/TH2 ratio decrease which indicates that the decreasing body immunity may be one of an important cause of TB infection.

关 键 词：结核 肺 T淋巴细胞 流式细胞术 斑点试验 

分 类 号：R446.6[医药卫生—诊断学]