一株兼性嗜冷产甲烷八叠球菌的分离鉴定及系统发育分析  

Isolation,Identification and Phylogenetic Analysis of an Amphipsychrophilic Methanosarcina

作　　者：耿钊 鲁建江[1] 童延斌[1] 汪晨霖 张硕[1] 孙世阳[1] 

机构地区：[1]石河子大学化学化工学院/新疆兵团化工绿色过程重点实验室/省部共建国家重点实验室培育基地,石河子832003

出　　处：《石河子大学学报（自然科学版）》2013年第5期645-650,共6页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(21067011);新疆兵团重点科技攻关计划项目(2010GG21)

摘　　要：为了提高低温条件下沼气发酵的效率，本文利用Hungate厌氧操作技术从新疆库尔勒越冬沼气池污泥中分离出一株生长温度范围为8～40℃的兼性嗜冷产甲烷菌株ZG-2。该菌株革兰氏染色为阳性，呈不规则球状叠放在一起，不运动，能够利用H。／C（]2混合物、甲醇、甲胺、乙酸盐作为单一碳源底物进行厌氧发酵生产甲烷。最适生长条件：温度为25～30℃，生长pH为6；NaCl浓度为0．2mol／L；不能耐受链霉素和卡那霉素。根据菌株形态特征和生理生化特性以及16SrDNA序列分析，初步认为菌株ZG-2为兼性嗜冷巴氏产甲烷八叠球菌（Methanosarcina barkeri）。In order to improve the efficiency of biogas fermentation under low temperature conditions, an amphipsychrophilic methanogen ZG-2 with growth temperature between 8 and 40~C was isolated by Hungate tube technique from the sludge of methane tank in Korla, Xinjiang, China. The cells of strain ZCr2 were aggregated, non-motile and gram-positive. This strain could use H2/CO2 mixture, ethanol, methylamine,dimethylamine or trimethylamine as the sole carbon source for its growth and methanogenesis. The optimum growth conditions were temperature of 25 to 30 ℃, pH 6.0 and NaCI concentration of 0.2 mol/ L. It was sensitive to streptomycin and Kanamycin. Morphological and physio-biochemical characteristics combined with 16S rD- NA sequence analysis demonstrated that ZG-2 belonged to Methanosarcina barkeri.

关 键 词：低温 厌氧发酵 兼性嗜冷产甲烷菌 分离鉴定 

分 类 号：S216.4[农业科学—农业机械化工程]