西黄丸对荷瘤大鼠的抗肿瘤及其对炎性因子的调节作用  

Antitumor activity of Xihuang Pill and its regulatory role of inflammatory cytokines

作　　者：杨伟[1] 关硕[1] 胡俊霞[1] 马杰[2] 曾常茜[1] 高文斌[3] 梁文波[1] 

机构地区：[1]大连大学医学院,辽宁大连116622 [2]遵义医学院,贵州遵义563000 [3]大连大学附属中山医院肿瘤科,辽宁大连116000

出　　处：《现代药物与临床》2013年第6期847-850,共4页Drugs & Clinic

摘　　要：目的研究西黄丸对Walker256乳腺癌细胞荷瘤大鼠的抗肿瘤及对相关炎性因子的调节作用,探讨其抗肿瘤作用机制。方法 60只雌性Wistar大鼠,随机抽取10只设为对照组,剩余50只建立荷瘤大鼠模型,随机分为模型组、西黄丸高、中、低剂量组和香菇多糖组,每组10只,按10 mL/kg ig给药,每天2次,连续14 d。14 d后腹主动脉取血,取瘤组织称定质量,计算抑瘤率;采用ELISA法检测大鼠外周血IL-2、IFN-γ、IL-6、IL-10和TGF-β水平。结果西黄丸高、中、低剂量组抑瘤率分别为33.1%、30.7%、28.5%;与对照组比较,西黄丸高、中、低剂量均可明显提高荷瘤大鼠外周血IL-2、IFN-γ的水平,降低IL-6、IL-1、GF-β水平(P<0.05)。结论西黄丸具有明显的抑制肿瘤生长的作用,可通过提高荷瘤大鼠IL-2、IFN-γ水平增强免疫清除功能;降低IL-6、IL-10、TGF-β水平,减少形成免疫抑制微环境的相关炎性因子,呈现出逆转肿瘤免疫逃逸的趋势。Objective To study the antitumor activity of Xihuang Pill on the growth of Walker 256 breast cancer cell in tumer-bearing rats and the regulatory role of the inflammatory cytokines of Xihuang Pill, and to explore its antitumor mechanism. Methods Ten of 60 female Wistar rats were seemed as control group and the remained 50 rats were established as tumor-bearing rat models which were randomly divided into five groups （each group with ten rats）： model group, high-, mid-dose, and low-dose Xihuang Pill, and lentinan groups. Each group was treatedt by ig administration twice daily for 14 d. After 14 d, the tumor was removed and weighed to calculate inhibitory rate. The levels of IL-2, IFN-γ, IL-6, IL-10 and TGF-β in peripheral blood were determined by ELISA. Results The tumor-inhibitory rates of high-, mid-, and low-dose Xihuang Pill groups were 33.1%, 30.7%, and 28.5%. Compared with the control group, the average levels of IL-2 and IFN-γ of the high-, mid-, and low-dose Xihuang Pill groups increased, and the levels of IL-6, IL-10 and TGF-β decreased （P 〈 0.05）. Conclusion Xihuang Pill has an obvious antitumor action which can enhance the expression of IL-2 and IFN-γ and reduce the expression of IL-6, IL-10, and TGF-β. The increase of the levels of IL-2 and IFN-γ could improve the immune clearance function and the decline of the levels of IL-6, IL-10 and TGF-β could reduce the expression of the related inflammatory factors in the immunosuppressive microenvironment which shows a trend to reverse tumor immune escape.

关 键 词：西黄丸 Walker256乳腺癌细胞 大鼠 炎性因子 免疫清除 免疫抑制微环境 

分 类 号：R285.5[医药卫生—中药学]