MhRAR1和MhSGT1基因转化苹果提高轮纹病菌诱导的抗氧化酶活性  

Transformation of Malus × domestica with MhRAR1 and MhSGT1 Genes from Malus hupehensis for Resistance of Botryosphaeria berengeriana

作　　者：屠煦童[1] 张仕杰[1] 吕东[1] 陈小云[1] 章镇[1] 渠慎春[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2013年第12期2354-2364,共11页Acta Horticulturae Sinica

基　　金：江苏省科技支撑计划项目(BE2011415)

摘　　要：RAR1和SGT1是植物R基因(抗病基因,resistance genes)介导的抗病防卫途径中的两个重要信号元件,在R蛋白下游和活性氧产生之间发挥作用。为了研究湖北海棠MhRAR1和MhSGT1基因在感病苹果品种植株内的表达特性及其抗病功能,利用农杆菌介导法将两种基因分别转入富士苹果。对Hyg阳性植株进行PCR和RT-PCR检测,6个转MhRAR1株系和3个转MhSGT1株系呈阳性;两种转基因苹果株系被苹果轮纹病病原菌侵染后,与对照相比都表现出抗氧化酶(SOD/POD/CAT)活性迅速升高、MDA含量变化较平缓的趋势,进一步说明RAR1和SGT1基因能够作用于苹果体内的活性氧反应和细胞膜活动进程,对植株受到外部侵害后的抗性反应有促进作用。RAR1 and SGT1 were identified as required components in some signaling pathways mediated by disease resistance （R）genes in many plant species, and they function between the downstream of R protein and reactive oxygen. To understand the expression characteristics and resistant function of MhRAR1 and MhSGT1 in susceptible variety, the two genes were transferred respectively into the Fuji apple by Agrobacterium tumefaciens-mediated method. After PCR and RT-PCR amplifications on Hyg positive plants, 6 MhRAR1 strains and 3 M＇hSGT1 strains were screened. Infected by Botryosphaeria berengeriana, the transgenic plants displayed more rapidly increase on all antioxidant enzymes （SOD/POD/CAT） activities and gentle changing waves of MDA content. It further illustrated that RAR1 and SGT1 act on the reaction of reactive oxygen species and the process of membrane reaction, as well as promote the resistant to external infringed reaction in apple.

关 键 词：苹果 湖北海棠 MhRAR1 MhSGT1 转基因 轮纹病 抗氧化酶活性 

分 类 号：S661.1[农业科学—果树学]