胡萝卜FLC同源基因对低温及光周期响应  

Expression Analysis of FLC Homologues Responding to Low Temperature and Photoperiod in Carrot

作　　者：毛笈华[1] 庄飞云[1] 欧承刚[1] 赵志伟[1] 王慧[1] 马振国[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京100081

出　　处：《园艺学报》2013年第12期2453-2462,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31272162);国家科技支撑计划项目(2012BAD02B04;2013BAD01B04);国家公益性行业(农业)科研专项(200903016)

摘　　要：基于‘松滋野生’(Ws)和‘Amsterdam’(Af)胡萝卜转录组测序,挖掘胡萝卜FLC同源基因(DcFLCs),深入研究其对低温及光周期响应规律。结果表明,胡萝卜中存在3个具有完整MADS-box和K-box保守结构域的FLC同源基因DcFLC1、DcFLC2、DcFLC3,分别编码209、212、219个氨基酸残基蛋白,进化树显示与其它植物FLC亲缘关系较远。RT-PCR表明DcFLC1和DcFLC2在供试材料中均表达,而DcFLC3仅在部分材料中表达。qPCR结果显示低温能促进DcFLC1和DcFLC3在耐抽薹品种Af幼苗叶片和种根中表达,而DcFLC2仅在Af种根中表达显著,在幼苗叶片中表达不显著。DcFLC1和DcFLC2在抽薹敏感的Ws幼苗叶片和种根春化过程中表达规律不同,低温能促进DcFLC1在幼苗叶片以及DcFLC2在种根中表达。连续光照能促进DcFLC1、DcFLC2在Af幼苗叶片中表达,但在Ws中则不同。Carrot（Daucus carom L. ）is a biennial species and requires vernalization for flowering. The premature bolting of carrot occurs in winter-spring plastic tunnel and spring cultivation, and results in a complete loss of commercial value, but limited progress reported on the control of bolting and flowering in carrot. Basis on the transcriptome sequences, carrot FLOWERING LOCUS C homologues （DcFLCs） were screened. Cultivar ＇ Amsterdam＇ （Af） tolerance to premature bolting and wild sensitive species ＇ Songzi＇ （Ws） were selected to study the relative expression of DcFLCs under cold and photoperiod treatment examined by real-time qPCR. Three FLC homologues （DcFLC1, DcFLC2 and DcFLC3） were annotated with complete ORFs, MADS-box and K-box conserved regions, which encoded 209, 212 and 219 amino acids, respectively. Phylogenetic analysis showed that DcFLCs were clustered with other plants FLC homologous. DcFLC1 and DcFLC2 were confirmed to express in all species by RT-.PCR, but DcFLC3 only in some. The relative expression of DcFLC1 and DcFLC3 were improved in Af young plants and roots during vernalization, while DcFLC2 was only improved in roots but no significance in young plants. DcFLC1 were improved in Ws young plants but no significance in roots during vernalization, while DcFLC2 in contrary. Both of the relative expression of DcFLC1 and DcFLC2 were improved in Af young plants under continuous illumination, but different in Ws.

关 键 词：胡萝卜 DcFLCs 低温 光周期 基因表达 

分 类 号：S631.2[农业科学—蔬菜学]