水仙红色副冠形成机理的初步研究  

Preliminary Studies on the Formation of Red Pigmentation in Corona of Narcissus

作　　者：郑益平[1] 吴雪琴[1] 曾黎辉[1] 

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《园艺学报》2013年第12期2479-2488,共10页Acta Horticulturae Sinica

基　　金：福建省自然科学基金项目(2011J01084)

摘　　要：以中国水仙(Narcissus tazetta var.chinensis)‘金盏银台’和喇叭水仙(N.pseudonarcissus L.)‘粉红魅力’为材料,通过定量PCR分析比较了类胡萝卜素合成途径主要结构基因在‘金盏银台’黄色副冠和‘粉红魅力’红色副冠中的表达;使用透射电镜观察‘粉红魅力’红色副冠以及‘金盏银台’黄色副冠、白色花瓣中有色体的超微结构;克隆了中国水仙有色体类胡萝卜素相关蛋白(CHRC)基因并分析其在不同组织中的表达。研究结果表明,‘粉红魅力’副冠呈现红色是一系列结构基因表达发生变化的结果,其中LYCE表达量降低是红色副冠中累积β–胡萝卜素的关键;红色和黄色副冠中有色体都为膜状有色体,副冠呈现红色与细胞中有色体的结构和数量无关,但红色副冠有色体中存储类胡萝卜素的质体球数量较多,这与其CHRC的表达量增强,有色体累积β–胡萝卜素的能力增强相关。研究还发现‘金盏银台’白色花瓣中有色体结构与其黄色副冠明显不同,有色体数量少,且有色体内质体球体积小、数量较少。研究结果将为阐明水仙花色形成机理以及利用基因工程技术改良中国水仙花色提供依据。Narcissus tazetta var. chinensis ＇ Jinzhan Yintai＇ and N. pseudonarcissus L. ＇ Pink Charm＇ were used as experimental materials in this study. The comparison of carotenoid biosynthetic gene expression between the yellow corona of ＇Jinzhan Yintai＇ and the red corona of ＇Pink Charm＇ was performed by real-time PCR. The ultrastructures of chromoplasts in red corona, yellow corona and white petal were investigated by transmission electron microscopy. In addition, the fall length cDNA of chromoplast-specific carotenoid-associated synthase （CHRC） was cloned from ＇ Jinzhan Yintai＇ by RACE method and the expression of CHRC was also analyzed. The results showed that the accumulation of abundant [3-carotenoid and the formation of red corona in ＇Pink Charm＇ were due to the difference in the expression of carotenoid biosynthetic genes between red and yellow corona, e：~pecially due to the decreasing of LYCE expression. Chromoplasts in yellow or red coronas were the same style with many homocentric membranes. The structure and amount of chromoplasts were not related to the red pigmentationformation. However, there were more numbers ofplastoglobules observed in chromoplasts of red corona, demonstrating that the red corona had the stronger capacity of pigment accumulation, which may be caused by the increasing expression of CHRC. The results also showed that the structure of chromoplasts in white petal of ＇Jinzhan Yintai＇ was quite different from its corona＇s, and plastoglobules were scarce and smaller in size. These results provide new insight into the mechanism of colour formation in Nacissus and the basis for the gene engineering of flower colour in Chinese narcissus.

关 键 词：水仙 红色副冠 类胡萝卜素 基因表达 有色体 CHRC 

分 类 号：S682.21[农业科学—观赏园艺]