萱草叶枯病菌生物学特性及对药剂敏感性研究  

Biological Characteristics and Fungicide Sensitivity of Kabatiella microsticta Causing Daylily Leaf Streak

作　　者：白庆荣[1] 韩双[1] 赵莹[1] 李海洋[1] 梁峻玮 高洁[1] 

机构地区：[1]吉林农业大学农学院,长春130118

出　　处：《园艺学报》2013年第12期2513-2519,共7页Acta Horticulturae Sinica

基　　金：大学生创新创业训练计划项目(201210193003)

摘　　要：针对中国新发现的萱草叶枯病病原菌Kabatiellamicrosticta的生物学特性和对药剂的敏感性进行了研究。结果表明：病菌菌丝生长和产孢适宜温度为25～30℃，最适温度28℃；菌丝生长最佳培养基为PDA、PSA和CA，产孢最佳培养基为V8汁培养基；D（＋）-麦芽糖和L-白氨酸分别为菌丝生长和产孢的最佳碳源和氮源；pH5～9适宜菌丝生长，pH7产孢最佳；光照对菌丝生长无影响，但有利于病菌产孢；病菌分生孢子的致死温度为49℃，10min。采用生长速率法测定了病菌对12种杀菌剂的敏感性：病菌对多菌灵、甲基硫菌灵、戊菌唑、丙环唑、氟硅唑、肟菌·戊唑醇、腈菌唑的敏感性较高，其EC50〈1．0mg·L-1，EC90〈5．0mg·L-1。本研究结果为研究病害发生规律及病害防治提供理论依据。Abstract： The biological characteristics and fungicide sensitivity of Kabatiella microsticta causing daylily leaf streak were studied. The results showed that the suitable temperature for mycelium growth and spore production of the pathogen was from 25 ℃ to 30 ℃, and 28 ℃ was the optimum. The optimal media for mycelium growth were PDA, PSA and CA, but V8 juice was the best for spore production. D （＋） - maltobiose and L-leucine were optimal for mycelium growth and spore production. The suitable pH for mycelium growth was 5 to 9, and 7 was optimum for spore production. Light c, ould promote spore production and had no effect on mycelium growth. The lethal temperature of conidia was 49 ℃, 10 min. The sensitivity ofK. microsticta to twelve fungicides was detected by mycelium growth rate method. The results showed that the pathogen was more sensitive to carbendazim, thiophanate-mel：hyl, penconazole, propiconazole, flusilazole, trifloxystrobin.tebuconazole, myclobutanil, EC50 〈 1.0 mg. L-1, EC90 〈 5.0 mg ·L1. This study will lay theoretical foundation for occurrence law and control of the disease.

关 键 词：萱草 叶枯病 生物学特性 药剂敏感性 

分 类 号：S682.19[农业科学—观赏园艺]