灰毡毛忍冬工厂化快繁生产体系的建立及再生苗遗传稳定性的分子鉴定  

Establishment of Rapid Propagation System and Molecular Identification of Genetic Stability on Lonicera macranthoides

作　　者：陈泽雄[1,2] 胡凯[1] 刘奕清[1,2] 

机构地区：[1]重庆文理学院林学与生命科学学院,重庆402160 [2]重庆市特色植物种苗工程技术研究中心,重庆402160

出　　处：《园艺学报》2013年第12期2520-2526,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31200512);重庆高校优秀成果转化项目(KJZH11220);重庆文理学院特色林木种质资源创新重点实验室平台建设;重庆市教委项目(KJ121210)

摘　　要：以灰毡毛忍冬(Lonicera macranthoides Hand.-Mazz.)腋芽为材料,建立了一套适合工厂化生产的离体再生技术体系。该体系包括腋芽诱导培养基MB+1.0 mg·L-16-BA+0.2 mg·L-1NAA,继代增殖培养基MB+1.0 mg·L-16-BA+0.5 mg·L-1IAA和MB+1.5 mg·L-16-BA+0.8 mg·L-1IAA和生根培养基1/2MB+2.0 mg·L-1IBA+0.2 mg·L-1IAA。分别从继代12、24、36个月的再生植株中随机抽取8株进行SRAP分析其遗传的变化。在检出的147条SRAP条带中,继代12个月的再生植株未发现变异,继代24个月的再生植株中有2株发生变异,继代36个月的再生植株中有4株发生变异。结果表明,建立的腋芽再生培养体系在一定继代周期内是稳定可靠的,适合灰毡毛忍冬的规模化生产。An efficient mass propagation system of Lonicera macranthoides Hand.-Mazz. was established based on axillary bud-derived medstems. The optimal media of tissue culture involving in the bud-induction medium （MB medium supplemented with 1.0 mg. L1 6-BA, 0.2 mg- LINAA）, subculture medium （MB medium supplemented with 1.0 mg· L-I 6-BA, 0.5 mg- L-11AAor 1.5 mg. L-1 6-BA, 0.8 mg · L-1 IAA） and root-induction medium （ 1/2MB medium supplemented with 2.0 mg · L-1 IBA and 0.2 mg· L-1 IAA） were selected. Thereafter, the genetic variation of 8 plants randomly sampled from regeneration plants cultured for 12, 24 and 36 months respectively was investigated by SRAP analysis and there were 0, 25% and 50% in variation rate found in regeneration lines respectively based on the 147 bands detected by SRAP markers. The results showed that regeneration culture system can maintain genetic stability during certain subculture cycles, indicating that it can be readily employed for large-scale propagation.

关 键 词：灰毡毛忍冬 快繁体系 遗传稳定性 分子鉴定 

分 类 号：S68[农业科学—观赏园艺]