Determination of epristeride by its quenching effect on the fluorescence of L-tryptophan  

作　　者：Ai-Qin Gong Xia-Shi Zhu 

机构地区：[1]College of Chemistry & Chemical Engineering,Yangzhou University [2]Yangzhou Polytechnic Institute

出　　处：《Journal of Pharmaceutical Analysis》2013年第6期415-420,共6页药物分析学报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China(20875082and 21155001);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Foundation of the Excellence Science and Technology Invention Team in Yangzhou University

摘　　要：A rapid, novel spectrofluorimetric method to determine epristeride （EP） in biological fluids and a pharmaceutical formulation was developed, based on the fact that fluorescence intensity of L-tryptophan could be quenched by EP in the medium of pH=9.0. The various factors influencing fluorescence quenching were discussed. The quenching mechanism was investigated with the quenching type, synchronous fluorescence spectra and quantum efficiency. Under the optimized conditions, fluorescence quenching value （AF^---FL_tryptophan--FEP_L_tryptophan） showed a good linear relationship with the EP concentration ranging from 0.4 to 12.0 lag/mL. The linearity, recovery and limit of detection demonstrated that the proposed method was suitable for EP determination in biological fluids and EP tablets. The method was successfully applied to the analysis of EP in real samples and the obtained results were in good agreement with the results of the official method.A rapid, novel spectrofluorimetric method to determine epristeride （EP） in biological fluids and a pharmaceutical formulation was developed, based on the fact that fluorescence intensity of L-tryptophan could be quenched by EP in the medium of pH=9.0. The various factors influencing fluorescence quenching were discussed. The quenching mechanism was investigated with the quenching type, synchronous fluorescence spectra and quantum efficiency. Under the optimized conditions, fluorescence quenching value （AF^---FL_tryptophan--FEP_L_tryptophan） showed a good linear relationship with the EP concentration ranging from 0.4 to 12.0 lag/mL. The linearity, recovery and limit of detection demonstrated that the proposed method was suitable for EP determination in biological fluids and EP tablets. The method was successfully applied to the analysis of EP in real samples and the obtained results were in good agreement with the results of the official method.

关 键 词：Epdsteride L-TRYPTOPHAN Fluorescence quenchingmethod 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.3[化学工程—制药化工]