炎症因子在阴道相关炎症性疾病中的局部表达  

Inflammatory Factors Expression in Vagina of Patients with Vaginal Related Inflammatory Diseases

作　　者：李桂军[1] 蒋琰瑛[1] 周建娟 蔡文伟[1] 

机构地区：[1]浙江省嘉兴市妇幼保健院,嘉兴314001

出　　处：《中国中西医结合皮肤性病学杂志》2013年第6期366-369,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：嘉兴市科技局计划项目(项目编号:2011AY1052-1)

摘　　要：目的探讨肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-8及IL-1β在细菌性阴道病(BV)、滴虫性阴道炎(TV)、外阴阴道念珠菌病(VVC)发病中的作用。方法 22例BV(BV组)、16例复发性BV(RBV组)、15例VVC(VVC1组)、6例TV(TV1组)患者及23例体检健康妇女(对照组)阴道分泌物镜检WBC<2个/HP;21例VVC(VVC2组)、18例TV(TV2组)患者阴道分泌物镜检白细胞计数(WBC)8~10个/HP。收集上述各组患者阴道灌洗液并离心。BV及RBV组患者每次治疗随访时均进行Nugent评分,收集其中治疗至Nugent评分4~6分(Nugent 4~6分组)患者阴道灌洗液21份及治疗至Nugent评分<3分(Nugent<3分组)患者阴道灌洗液17份并离心。酶联免疫吸附试验(ELISA)方法检测阴道灌洗液上清液中各因子浓度;沉淀提取总核糖核酸(RNA),荧光定量聚合酶链反应(PCR)法检测各因子mRNA。结果与对照组相比,VVC2、TV2组4种因子浓度及mRNA表达均明显上调;VVC1组中TNF-α及IL-1β、TV1组的IL-8浓度及mRNA表达也明显上调;其他组各因子及mRNA表达变化均不显著。结论 4种炎症因子在BV发病中的作用并不显著。IL-8、IL-1β、TNF-α参与TV、VVC发病。BV表现非炎症反应与阴道局部IL-8、IL-1β、TNF-α等低表达有关。TV常伴严重炎症反应与阴道局部IL-8高表达有关。Objective To evaluate the effect of TNF-c~, IL-1 [3, IL-8 and IL-6 on the pathogenesis of BV, VVC and TV. Methods 22 BV patients （BY group）, 16 RBV patients （RBV group）, 15 VVC patients （VVC 1 group）, 6 TV patients （FVl group） and 23 healthy women （control group） whose WBC were less than 2/HP in vaginal secretion and patients of 21 VVC （VVC2 group）, 18 TV （TV2 group） whose WBC were 8 to IO/HP in vaginal secretion were recruited into this study. Vaginal lavage fluid was collected and centrifuged at the first visit. At the second and following-up visit, Nugent score of BV and RBV patients was detected, 21 vaginal lavage fluid of patients whose Nugent were four to six score （Nugent four to six score group） and 17 vaginal lavage fluid of patients whose Nugent were less than three score （Nugent less than three score group） were collected. The concentration of inflammatory factors in vaginal lavage fluid was measured by enzyme-linked immunosorbent assay （ELISA） and mRNA expression in vaginal epithelial cells was analyzed by fluorescent quantitation PCR. Results Compared with control group, four inflammatory factors concentration and mRNA expression on VVC2 group and TV2 group increased significantly, concentration and mRNA expression of TNF-ct, IL-1 ~ in VVC1 and IL-8 in TV1 increased significantly too. Conclusion There is no strong relationship between TNF-c~, IL-l~, IL-8, IL-6 and BV. Inflammatory factors such as IL-8, IL-l^3 and TNF-ct play important effect on VVC and TV pathogenesis. Clinical situation of BV patients with no inflammatory reaction is related to IL-8,IL-I[3 and TNF-ct low expression in vaginal mucosa, TV patients with inflammatory reaction is relate to high IL-8 expression in vaginal mucosa.

关 键 词：细菌性阴道病 外阴阴道念珠菌病 滴虫性阴道炎 免疫 

分 类 号：R711.31[医药卫生—妇产科学]