脑心肌炎病毒实验性疫苗免疫原性初步研究  

Preliminary study on the immunogenicity of an experimental encephalomyocarditis virus vaccine

作　　者：徐静[1] 李树香[1] 刘敬[1] 王欣怡[1] 岳野[1] 凌媛[1] 

机构地区：[1]北京生物制品研究所有限责任公司第三研究室,101111

出　　处：《中华微生物学和免疫学杂志》2013年第12期950-953,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的：制备脑心肌炎病毒（ EMCV）实验性疫苗并对其免疫原性进行初步研究。方法采用Vero细胞培养EMCV病毒，微量细胞病变法检测病毒滴度；经过浓缩、过滤、超速离心等方法初步纯化EMCV；摸索病毒灭活方法；确定病毒半数致死量（ LD50）；通过肌肉注射途径免疫小鼠，微量中和试验测定血清中和抗体效价；免疫后小鼠分别以100LD50、50LD50和25LD50进行腹腔攻毒试验。结果以1∶4000β-丙内酯4℃放置12 h作为病毒灭活条件，病毒半数致死量17 CCID50/ml；免疫小鼠能检测到中和抗体并对病毒攻击具有抵抗力。结论 EMCV实验性疫苗能诱导小鼠产生保护性抗体。Objective To prepare an experimental encephalomyocarditis virus （ EMCV） vaccine and study its immunogenicity .Methods EMCVs were cultured in Vero cells and viral titer was detected by micro cytopathy method .A series of procedures including concentration , filtration and ultracentrifugation were conducted to purify EMCVs .Formaldehyde and β-propiolactone were used for viral inactivation and their efficacy was evaluated .The median lethal dose （ LD50 ） of EMCV was measured by using Reed-Muench method.The serum antibody titers in BALB/c mice vaccinated with different immunization strategies were analyzed through microneutralization assay .Then the immunized mice were challenged with 100LD50 , 50LD50 and 25LD50 of EMCV by intraperitoneal injection .Results The viruses could be inactivated by β-propiolactone at a volume ratio of 1 ∶4000 for 12 hours at 4℃.The LD50 of EMCV was 17 CCID50/ml.The immunized mice produced neutralizing antibodies and displayed resistance to EMCV infection.Conclusion The experimental EMCV vaccine could induce protective antibodies in mice .

关 键 词：脑心肌炎病毒 免疫原性 半数致死量 

分 类 号：R186[医药卫生—流行病学]