白背飞虱体内RNA干扰技术体系的建立  

Establishment of RNA interference system in white-backed planthopper

作　　者：贾东升[1] 任堂雨[1] 陈红燕[1] 魏太云[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2013年第6期579-583,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金重点项目(3113044);福建省杰出青年科学基金项目(2011J06008);教育部高等学校博士学科点专项科研基金(20113515130002);863计划(2012AA101505)

摘　　要：应用双链RNA(dsRNA)诱导的RNA干扰(RNAi)技术原理,将体外合成的源于白背飞虱肌动蛋白(Actin)基因的dsRNA通过膜饲喂法饲喂其2龄若虫.通过优化人工饲料、dsRNA浓度和饲喂时间,成功地干扰了白背飞虱Actin基因的表达,并引发白背飞虱的死亡,从而建立了白背飞虱体内的RNAi体系.The potential of RNA interference （RNAi） induced by double-strand RNA （dsRNA） was tested in white-backed plan- thopper （WBPH）. dsRNAs corresponding to the Actin gene of WBPH were produced in vitro and applied to the 2nd instar nymphs of WBPH through a membrane-feeding method. After optimizing the artificial diet, dsRNA concentration and feeding time, the ex- pression of Actin in cells of WBPH was significantly reduced, leading to death of the insects prematurely. This indicated that the in vivo RNAI system for WBPHs was established.

关 键 词：白背飞虱 RNA干扰 ACTIN基因 双链RNA 膜饲喂法 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]