煅烧牛骨对人牙髓干细胞体外增殖、牙向分化能力的影响  

Effects of Calcined Bovine Bone on Proliferation and Odontogenic Differentiation of Human Dental Pulp Stem Cells in Vitro

作　　者：张红梅[1] 荀文兴[1] 张晓梅[2] 王江[3] 李蓉[1] 

机构地区：[1]第四军医大学唐都医院口腔科,陕西西安710038 [2]解放军总医院消化内科 [3]第四军医大学西京医院神经外科

出　　处：《口腔医学研究》2013年第12期1131-1134,共4页Journal of Oral Science Research

摘　　要：目的:观察煅烧牛骨(calcined bovine bone,CBB)与人牙髓干细胞(human dental pulp stem cells,hDPSCs)的生物相容性及其作为支架材料的牙向诱导作用。方法:体外分离培养DPSCs,分别用四唑盐比色法(MTS)、茜素红染色法、碱性磷酸酶(alkaline phosphatase,ALP)和逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)方法观察CBB生物材料对DPSCs增殖活性和牙向分化能力的影响。结果:CBB显著地刺激体外培养的DPSCs增殖,诱导了细胞的矿化和提高细胞ALP活性。RT-PCR结果显示CBB诱导后细胞mRNA水平表达牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)和牙本质基质蛋白(dentin matrix protein1,DMP-1)。结论:体外培养条件下,CBB与DPSCs有良好的生物相容性,作为支架材料对于DPSCs有较好牙向诱导作用。Objective： To observe the adhesion and growth of human dental pulp stem cells seeded on calcined bo vine bone （CBB） in vitro and the odontogenic induction of CBB. Methods： DPSCs were successfully isolated and cul tured. The compatibility of the biomaterial was evaluated by MTS assay. The odontogenic differentiation of DPSCs induced by CBB was measured using Alizarin red staining, the alkaline phosphatase （ALP） activity and reverse trar scription-polymerase chain reaction （RT-PCR） assay. Results： The proliferation of DPSCs in CBB groups was obviously higher than that in the control groups. The higher levels of ALP activity and larger mineralizing nods were also detected in the experiment groups. RT-PCR demonstrated mRNA expression of dentin sialophosphoprotein （DSPP） and dentin matrix proteinl （DMP-1） in DPSCs induced by CBB. Conclusion： CBB possesses a good cellu lar compatibility with DPSCs. As an engineering scaffold, it can induce the odontogenic differentiation of DPSCs.

关 键 词：牙髓干细胞 煅烧骨 增殖 分化 

分 类 号：R783.5[医药卫生—口腔医学]