肠粘连引起肠梗阻的基因水平研究  

Study of Intestinal Obstruction Caused by Intestinal Adhesion on The Gene Level

作　　者：颜禄斌[1] 崔鸿斌[1] 李斌德[1] 李来元[1] 李刚[1] 邴志桐[2] 沈阳[1] 

机构地区：[1]兰州大学第二医院普外科,甘肃兰州730030 [2]中国科学院近代物理研究所,甘肃兰州730000

出　　处：《中国普外基础与临床杂志》2013年第12期1369-1373,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：甘肃省青年基金资助项目(项目编号:1208RJYA013);甘肃省中医药管理局重点科研基金项目(项目编号:GSWS2008)~~

摘　　要：目的通过分析基因层面的差异表达，以探讨与肠梗阻相关的发病基因和治疗靶基因。方法在基因表达公共数据库（geneexpressionomnibus，GEO）中收集到10个样品的基因表达数据，其中小鼠粘连小肠组织术后1、3、7、和14d时间点的基因芯片数据共8个，小鼠正常小肠组织芯片数据2个，应用基因本体论（geneontology，GO）富集分析和微阵列显著性分析（significanceanalysisofmicroarray，SAM）方法，分析10个样品的基因表达情况，并对差异表达的基因进行功能注释和生物学分析。结果肠粘连的发生伴随着大量应答刺激的基因表达上调，且这些基因产物都分布在细胞膜上。分析术后不同时间点的基因表达情况发现，Hmgcs2基因的表达上调出现在术后3～14d，融助5基因的表达上调出现在术后14d。结论粘连的发生可能与应答刺激的基因及其分布于细胞膜上的基因产物有关，Hmgcs2和s抚幼5基因可能与因粘连而导致的其他并发疾病的发生有关。这为发现肠梗阻潜在的治疗靶点提供了依据。Objective To explore the pathogenesis of the level ofgene and therapeutic target genes associated with intestinal obstruction by analyzing the differential expression gene. Methods The gene expression data that came from public database gene expression omnibus （GEO） which provided adhesion formation＇ gene expression data on 1, 3, 7, and 14 days after operation （n=8） and normal intestinal tissues＇ gene expression data （n=2） of mouse were collected. The gene function and differential expression of genes were analyzed by using gene ontology （GO） and significance analysis ofmicroarray （SAM）. Results There were a lot of response stimulated up-regulation ofgene expression when occurrence of adhesion, and the products of these genes were distributed on cell membrane. The analysis results of gene expression at different time point after operation showed that expression up-regulated ofHmgcs 2 gene occurred on 3-14 days ofter operation and expression up-regulated of Stxbp 5 gene occurred on 14 days ofter operation. Conclusions The adhesion formation may be closely associated with the genes of response to stimulus and the gene product in membrane. The Hmgcs 2 and Stxbp 5 genes may be closely associated with the occurrence of other diseases which induced by adhesion formation. This provides a basis for the discovery of potential therapeutic targets.

关 键 词：肠梗阻 基因芯片 基因本体论分析 基因表达数据库 

分 类 号：R656.7[医药卫生—外科学]