pokemon蛋白在胃上部癌组织中的表达对判断外科分子切缘的意义  

Expression of Pokemon Protein Predicts The Surgical Molecular Margin in Upper Gastric Cancer

作　　者：杨卫[1] 蔡新新 曲宏岩[1] 李岩峰[1] 张永乐[1] 

机构地区：[1]哈尔滨医科大学附属第三医院胃肠外科,黑龙江哈尔滨150081 [2]桂林医学院研究生学院麻醉学系,广西桂林514000

出　　处：《中国普外基础与临床杂志》2013年第12期1386-1390,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：黑龙江省教育厅科学技术研究项目(项目编号:12531354)~~

摘　　要：目的探讨以pokemon蛋白判断胃上部癌手术切缘的安全距离。方法对60例胃上部癌，在肿瘤中心以及距上下切缘1．0、1．5、2．0、2．5、3．0、3．5、4．0、4．5及5．0cm处分别取材。采用Westernblot法检测pokemon蛋白的表达情况。结果60例胃癌组织中，pokemon蛋白表达阳性38例，表达阳性率为63．3％。与肿瘤中心处比较，上下切缘距离为1．0～2．0cm处组织中pokemon蛋白表达阳性率的差异均无统计学意义（P≥0．003），而2．5～5．0cm处组织中pokemon蛋白的表达阳性率均较低（P〈0．003）。上下切缘距离达3．5cm及以上处组织中pokemon蛋白的表达阳性率均为0。上下切缘对应位置pokemon蛋白的表达阳性率比较差异均无统计学意义（P〉0．05）。结论以pokemon蛋白的表达情况作为对胃上部癌组织切缘的研究依据，结果提示3．5cm及以外的组织pokemon蛋白的表达与正常组织无差异，可能对外科手术切缘的确定具有一定指导意义。Objective To investigate the safe distance of upper gastric cancer by pokemon protein. Methods In 60 cases of upper gastric cancer, specimens were get from tumors＇ tissue and every 0, 5 cm from the proximal of tumor margin beginning at the 1.0 cm to distal. Western blot method was used to test the expression ofpokemon protein. Results There were 38 cases （63.3%） positively expressed pokemon protein. Compared with the center of tumor, the positive expression rates of pokemon protein at every sites of 2.5-5.0 cm from margin were lower （P〈 O. 003）, but didn＇t signi- ficantly differed at every sites of 1.0 2.0 cm from margin （P~〉0. 003）. When the incisal margin distance of more than 3.5 cm of both sides, the positive expression rate of pokemon protein were 0. There were no significant difference between the corresponding sites of the upper resection margin and lower resection margin （P 〉 0. 05）. Conclusions The results show that 3.5 cm may be the molecular margin basing on the positive expression ofpokemon protein in the surgical margin of upper gastric cancer, which may have guiding significance to the surgical margin.

关 键 词：POK红系髓性致癌因子 胃上部癌 分子切缘 

分 类 号：R656.61[医药卫生—外科学] R735.2[医药卫生—临床医学]