春石斛优良品种‘森禾2006’组培快繁体系的建立  

Establishment of Rapid Propagation System for Elite Variety of Dendrobium nobile Lindl. ‘Senhe 2006' by Tissue Culture

作　　者：贾梦雪[1] 徐瑾[1] 叶香娟 刘芊[1] 王喆 刘燕[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术研究中心,北京100083 [2]浙江森禾种业股份有限公司,杭州310012

出　　处：《植物生理学报》2013年第12期1363-1367,共5页Plant Physiology Journal

基　　金：国家“十二五”支撑计划课题(2011BAD12B02-01)

摘　　要：本文研究了春石斛‘森禾2006’的拟原球茎(PLBs)诱导、增殖、分化及壮苗和生根,建立了其组培快繁体系。结果表明:‘森禾2006’PLBs最适诱导培养基为1/2MS+TDZ 0.5 mg·L-1+水解酪蛋白2.0 g·L-1;PLBs增殖培养基为1/2MS+水解酪蛋白2.0 g·L-1;PLBs分化培养基为1/2MS+KT 1.0 mg·L-1+NAA 0.1 mg·L-1;壮苗生根培养基为MS+IBA 0.5 mg·L-1+NAA 0.1 mg·L-1+香蕉泥100.0 g·L-1。To achieve the mass-scale multiplication of Dendrobium nobile ＇Senhe 2006＇, the optimal media for the induction, multiplication and differentiation of protocorm-like bodies （PLBs）, strengthening and rooting were studied and the rapid propagation system by tissue culture was established. The results showed that I/2MS＋TDZ 0.5 mg.L%casein hydrolysate 2.0 g.L1 was optimal for PLB induction. 1/2MS＋casein hydrolysate 2.0 g.L-1 was the most suitable for PLB multiplication. 1/2MS＋KT 1.0 mg.L-1＋NAA 0.1 mg-L-1 was the most efficient for PLB differentiation. The optimum medium for strengthening and rooting was MS＋IBA 0.5 mg.L-1 ＋NAA 0.1 mg.L-l＋banana homogenate 100.0 g.L-1.

关 键 词：春石斛 拟原球茎(PLBs) 组培快繁 种苗 

分 类 号：S682.31[农业科学—观赏园艺]