化学诱变剂和^(60)Co处理对大麦小孢子低氮胁迫培养的影响  

Effects of Chemical Mutagen and ^(60)Co Treatments on Microspore Culture of Barley under Low-Nitrogen Stress

作　　者：陆瑞菊[1,2] 陈志伟[1,2] 何婷[1,2] 徐红卫[1,2] 杜志钊[1,2] 高润红[1,2] 王亦菲[1,2] 邹磊[1,2] 郭桂梅[1,2] 卜姝明[3] 黄亦辰[3] 刘成洪[1,2] 黄剑华[1,2] 

机构地区：[1]上海市农业科学院生物技术研究所,上海201106 [2]上海市农业遗传育种重点实验室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《植物生理学报》2013年第12期1442-1446,共5页Plant Physiology Journal

基　　金：大麦青稞产业技术体系(CARS-05);上海市种业发展项目[沪农科种字(2012)第7号];上海市科委基础重点项目(12JC1407800)

摘　　要：以大麦品种‘花30’为材料,用不同剂量化学诱变剂(EMS和PYM)处理大麦游离小孢子,不同剂量60Co辐照处理大麦离体穗和干种子,比较其对在低氮胁迫下小孢子培养诱导愈伤组织产量和绿苗分化数量的影响。结果表明,EMS处理离体小孢子和60Co辐照干种子明显比PYM处理小孢子和60Co辐照离体穗的培养效果好。To investigate the effects of chemical mutagens and 60Co on callus yield and green plantlet production through microspore culture under low-nitrogen stress, we used the barley （Hordeum vulgare） variety ＇Hua 30＇ as the tested material, used two chemical mutagens, ethylmethylsulfone （EMS） and pingyangmycin （PYM）, with different concentrations to treat the microspores, and used 60C0 with different doses to treat young spikes and seeds respectively. The results showed that EMS treatment of microspores or 60Co treatment of seeds were much better than PYM treatment of microspores or 60Co treatment of young spikes for callus induction and green plantlet differentiation.

关 键 词：大麦 小孢子培养 诱变 

分 类 号：S512.3[农业科学—作物学]