左旋聚乳酸/壳聚糖/羟基磷灰石复合仿生支架修复骨缺损的实验  

The experimental study of repairing large bone defect with biomimetic poly(L-latic acid) /chitosan / hydroxylapatite composite scaffolds

作　　者：郇松玮[1] 姚平[2] 刘宁[1] 赵剑豪[3] 吴昊[1] 黄浩[1] 查振刚[1] 

机构地区：[1]暨南大学附属第一医院骨科,广东广州510630 [2]暨南大学医学院,广东广州510632 [3]暨南大学理工学院,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2013年第6期593-598,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省科技计划项目(2011B050300013);澳门科学技术发展基金项目(025/2010/A);广东省中医药管理局基金项目(20111174)

摘　　要：目的:评价左旋聚乳酸/壳聚糖纳米纤维/纳米羟基磷灰石(poly(L-latic acid)/chitosan nano-fibers/nano-hydroxylapatite,PLLA/CSNF/nHA)复合仿生支架联合骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)修复新西兰兔桡骨大段缺损的可行性。方法:二次相分离法制作PLLA/CSNF/nHA仿生支架,并与新西兰兔的3代BMSCs复合。制作新西兰兔桡骨1.5 cm段状缺损模型27只,实验组缺损处植入PLLA/CSNF/nHA/BMSCs复合物、对照组植入PLLA/CSNF/nHA仿生支架、空白组不做处理,于术后第4、10、16周通过大体形态观察、放射学检查和组织学检查等方法评价缺损修复情况。结果:术后末次观察,实验组已完成缺损修复,材料完全降解;对照组部分修复,仍残留部分缺损;空白组缺损主要由纤维结缔组织填充。结论:PLLA/CSNF/nHA复合支架的仿生结构具有良好的生物相容性,联合BMSCs在生物体内可以加快新骨生成,完成新西兰兔大段骨缺损的修复。Aim：To evaluate the feasibility of the biomimetic poly （L-latic acid ）/chitosan nano-fi-bers/nano-hydroxylapatite （PLLA/CSNF/nHA）composite scaffolds combined with bone marrow mesen-chymal stem cells （BMSCs）to repair bone defect of New Zealand rabbits.Methods：PLLA/CSNF/nHA composite scaffolds were prepared through the dual phase separation technique,then co-cultured with BM-SCs.The large defects （1.5 cm）in radius of rabbits were prepared and experimented in three groups. PLLA/CSNF/nHA/BMSCs compound was planted into the defected position in the experimental group;PLLA/CSNF/nHA scaffolds in the control;and no implant in the blank.The curative effect was evalua-ted by general -observation,radiology examination and HE staining at the 4th,1 0th and 1 6th week.Re-sults：The defects were repaired perfectly with the scaffolds degraded in the experimental group;partially repaired and degraded in the control;and full of fibrillar connective tissues in the blank,by the last eval-uation.Conclusion：PLLA/CSNF/nHA biomimetic scaffolds composited with BMSCs of rabbits have a good role to accelerate the formation of new bone and repair the large bone defect.

关 键 词：骨髓间充质干细胞 骨缺损 壳聚糖 左旋聚乳酸 仿生支架 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]