乳化氟碳保存液对离体保存肾脏抗氧化能力及组织结构的影响  

The influence of oxygenated perfluorocarbon preservation solutions on antioxidation and histological structure of donor kidney

作　　者：彭雪梅[1] 刘嘉羿 郑志雄[1] 邹启荣[1] 谢娟华[1] 席露[2] 刘慧慧[3] 

机构地区：[1]暨南大学第一附属医院麻醉科,广东广州510630 [2]广州医学院第三附属医院麻醉科,广东广州510150 [3]广州中医药大学第一附属医院麻醉科,广东广州510405

出　　处：《暨南大学学报（自然科学与医学版）》2013年第6期599-603,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省科技计划项目(2060303);广东省医学科研基金项目(A2010338);广东省中医药局科研基金项目(20132115)

摘　　要：目的：观察乳化氟碳保存液和高渗枸橼酸盐嘌呤溶液对低温保存肾脏抗氧化能力和超微结构的影响，探讨乳化氟碳保存液在供体肾脏保存中的作用。方法：选择24只雄性SD大鼠随机分为高渗枸橼酸盐嘌呤溶液组（HCA）和乳化氟碳液组，每组12只。分别采用乳化氟碳保存液和高渗枸橼酸盐嘌呤溶液原位灌注双侧肾脏后快速切取，置于0～4℃20 mL上述相应液体中保存24 h后，比较两组保存液对大鼠肾脏的肾皮质匀浆MDA含量、SOD活性及Bcl-2基因表达和形态学改变。结果：乳化氟碳液组肾皮质匀浆MDA含量明显低于HCA液组（P＜0.05），SOD活性、Bcl-2基因表达明显高于HCA液组（P＜0.05），病理评分低于HCA液组（P＜0.05），肾小管上皮细胞损伤轻于HCA液组。结论：乳化氟碳液可提高供体肾抗氧化和抗凋亡能力，减轻供体肾保存期的肾损伤。Aim：To study renal protective effect of the cryopreservation with oxygenated perfluorocar-bon preservation solutions.Methods：Male SD rats were randomly divided into （hypertonic citrate ade-nine）HCA and （perfluorocarbon emulsion）PFCE groups with twelve rats in each group.The kidney was infused in situ with the corresponding HCA solution and PFCE,respectively,then harvested and pre-served at 0~4℃in 20 ml of liquids for 24 hours.The levels of MDA,SOD activity and Bcl-2 protein as well as morphological changes were determined.Results：The renal MDA content was significantly lower in PFCE group than in HCA group （P〈0.05 ）,SOD activity and Bcl-2 expression were significantly higher in PFCE group than HCA group （P〈0.05 ）,pathological scores were lower in PFCE group than HCA group （P〈0.05 ）.Futhermore,PFCE reduced renal tubular epithelial cell injury compared with＆nbsp;HCA solution.Conclusion：Perfluororcarbon emulsions can improve antioxidant and anti-apoptosis ability of kidney to reduce renal damage during preservation of donor kidney.

关 键 词：冷保存 氟碳化合物 BCL-2 

分 类 号：R816[医药卫生—放射医学]