乙醇对简单节杆菌生长、生理特性和细胞结构的影响  

Influence of Ethanol on the Growth,Physiological Characteristics and Cell Structure of Arthrobacter Simplex

作　　者：宁静 刘莉娜 王敏[1] 骆健美[1] 

机构地区：[1]工业发酵微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2013年第6期9-14,19,共7页Journal of Tianjin University of Science & Technology

基　　金：天津市应用基础与前沿技术研究计划(13JCYBJC20600);天津科技大学实验室开放基金科技活动开放类课题(1204A210)

摘　　要：分析简单节杆菌乙醇耐性差异菌株的生长特性，以静息细胞为对象，考察乙醇处理对菌体细胞存活率的影响，进一步分析细胞糖代谢能力、细胞膜电位、细胞超显微结构和通透性的变化．结果发现：乙醇体积分数在0％。10％的范围内，突变菌株UV15X1．2比出发菌株TCCC11037表现出更好的生长特性；6％和10％乙醇处理后，突变菌株UV15X1—2的细胞存活率比出发菌株TCCC11037分别高出19．2％和18．3％，表现出更好的乙醇耐受性；进一步研究发现，6％乙醇引起出发菌株TCCC11037糖代谢能力和膜电位的明显下降，细胞完整性遭到明显破坏，而对于突变菌株UV15X1．2，这些变化在乙醇体积分数为10％的条件下出现．此外，通过测定FDA的相对荧光强度和细胞蛋白质及核酸的泄漏量，表明一定体积分数的乙醇处理能显著提高简单节杆菌的细胞通透性．The growing properties of Arthrobacter Simplex strains with different organic solvent tolerance were studied. Using resting cells as materials, the effects of ethanol on the cell survival, sugar metabolic activity, membrane potential, cell ultrastructure and permeability were investigated. The results showed that the growing properties of UV15X1-2 were better than those of TCCC 11037 within the range of ethanol concentration （0%-10%） . After the treatment with 6% and 10% of ethanol, the cell survival rate of UV15X1-2 was 19.2% and 18.3% higher compared with TCCC 11037, which suggested that UV15XI-2 presented higher tolerance toward ethanol. As for TCCC 11037, sugar metabolic activities and membrane potential decreased rapidly, and the cell integrity was significantly damaged with 6% of ethanol, but similar effects on cell properties and structure were not observed until UV15X1-2 was treated with 10% of ethanol. On the other hand, the relative fluorescence intensity of FDA and the protein/nucleic acid leakage revealed that ethanol could enhance the permeability of the Arthrobacter simplex cell significantly.

关 键 词：简单节杆菌 乙醇 细胞结构 有机溶剂耐受性 

分 类 号：Q93[生物学—微生物学]