氯诺昔康乳膏剂的制备和含量测定  

Preparation and content determination of the lornoxicam cream

作　　者：金伟华[1] 范开华[1] 于波涛[1] 陈华[1] 易荣[1] 

机构地区：[1]成都军区总医院药剂科,成都610083

出　　处：《药学服务与研究》2013年第6期460-462,共3页Pharmaceutical Care and Research

摘　　要：目的：制备氯诺昔康乳膏剂，建立HPLC法测定乳膏剂中氯诺昔康的含量。方法：用水包油型基质制备含氯诺昔康0.2%的乳膏剂，HPLC法所用色谱柱为Eclipse XDB-C18柱，流动相为0.05 mo1/L乙酸钠溶液（pH 6.5）-甲醇（50∶50），流速1 ml/min，检测波长378 nm，柱温30 ℃。结果：氯诺昔康在0.01～0.10 mg/ml范围内呈良好线性关系，标准曲线方程为：A=4.85×104 c-29.69（r=0.999 5）。低、中、高浓度（0.02、0.06、0.10 mg/ml）氯诺昔康的加样回收率分别为（99.10±1.37）%、（101.47±0.37）%和（99.25%±1.89）%（n=3），日内和日间精密度良好。结论：制备的氯诺昔康乳膏剂质量合格，建立的含量测定方法简便、准确、可靠。Objective：To prepare lornoxicam cream and establish a HPLC method for the determination of the content of lornoxicam. Methods：The cream containing 0.2% lornoxicam was prepared by using oil in-water matrix. HPLC was applied on an Eclipse XDB-C18 column with the mobile phase consisting of 0.05 mol/L sodium acetate solution （pH 6.5）-methanol（50 ： 50）. The flow rate was i ml/min and detection wavelength was 378 nm and the column temperature was 30 C. Results：Lornoxi cam was of good linearity within the range of 0.01-0. 10 mg/ml. The standard curve equation was A = 4.85 × 101 c 29.69（r=0. 999 5）. The recovery rates of low-,middle and high concentrations（0.02,0. 06,0. 10 mg/ml） were （99.10±1. 37）%, （101.47±0.37） % and （99.25± 1.89）% （n = 3）. The precision of intra-day and interday was also good. Conclusion.. The prepared lornoxieam cream met with specified quality and the HPLC method for content determination was simple,accurate and reliable.

关 键 词：氯诺昔康 乳膏剂 含量测定 色谱法 高效液相 

分 类 号：R971.1[医药卫生—药品] R944.21[医药卫生—药学]