日本落叶松体细胞胚胎发生相关基因LaSERK1的克隆与表达分析  

Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1(SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

作　　者：李龙[1] 张立峰[2] 齐力旺[2] 韩素英[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,北京100091 [2]中国林业科学研究院林业研究所细胞生物学实验室,北京100091

出　　处：《林业科学研究》2013年第6期673-680,共8页Forest Research

基　　金：国家重点基础研究发展计划(973)项目"速生优质林木培育的遗传基础及分子调控"(2009CB119100);国家高技术研究发展计划(863)项目"高产优质多抗林木花草分子与细胞高效育种技术及品种创制"(2006AA100109)

摘　　要：本研究克隆了日本落叶松体细胞胚胎发生相关受体类蛋白激酶基因LaSERK1,并检测了在不同培养条件下LaSERK1的表达情况,发现LaSERK1与已发现的其他物种SERK1基因有高度相似性,qRT-PCR结果显示:LaSERK1在体胚发生早期高表达。结果表明:LaSERK1可能在落叶松体胚发育早期起重要作用,LaSERK1也可能成为早期胚性细胞的标记基因。Somatic embryogenesis not only plays a significant role in understanding the mechanism of embryogene- sis, but also in improving the breeding of Larix kaempferi （Lamb.） Carr. The somatic embryogenesis receptor-like kinase 1 （SERK1） gene is considered as an essential element involved in the early stage of this process. The ex- pression pattern of the homolog of SERK! of L. kaempferi in different culture conditions was studied. LaSERK1 shared all the characteristic domains of the SERK family. The qRT-PCR showed that LaSERK1 was highly expressed in L. kaempferi early embryogenic cells cultured in the mature medium. These results suggest that LaSERK1 might perform a significant role during early somatic embryogenesis of L. kaempferi, and might be a potential marker of ear- ly embryogenic cells.

关 键 词：日本落叶松 体细胞胚胎发生 SERK1 表达分析 

分 类 号：S718.46[农业科学—林学]