山萘酚对脂多糖诱导RAW264-7细胞中COX-2和iNOS表达及产物生成的影响  

The effect of kaempferol on COX-2and iNOS expression and generated production in LPS-induced RAW 264.7 cells

作　　者：陈美珺[1] 金宏伟[1] 陈振胜[1] 黄回滨[1] 梁统[2] 周克元[2] 

机构地区：[1]厦门大学附属中山医院临检中心,福建厦门361005 [2]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《沈阳药科大学学报》2013年第12期982-985,990,共5页Journal of Shenyang Pharmaceutical University

基　　金：广东省社会发展领域科技计划项目(53025);广东省重点扶持学科项目(GX0307)

摘　　要：目的探讨山萘酚对脂多糖(lipopolysaccharides,LPS)诱导RAW 264.7细胞COX-2及iNOS表达的影响。方法四氮唑盐法(monote-trazolium test,MTT)检测山奈酚对RAW264.7细胞生长增殖的影响,放射免疫测定法(RIA)检测山萘酚对PGE2和NO生成的影响,免疫印迹法(western blotting)检测COX-2及iNOS蛋白的表达。结果山萘酚抑制LPS诱导的RAW 264.7细胞PGE2和NO的生成,同时下调LPS诱导的RAW 264.7细胞COX-2及iNOS蛋白的表达。结论山萘酚抑制2个诱导酶COX-2和iNOS的表达,从而减少炎性产物PGE2和NO的生成,这可能是山萘酚抗炎的机制之一。Objective To study the effect of kaempferol on COX-2 and iNOS expression in LPS-induced RAW 264.7 cells. Methods The effects of kaempferol on the proliferation in RAW264.7 cells were evalua- ted by monote-trazolium test assay （MTT） ;the effect o f kaempferol on the product of PGE2 and NO in RAW264. 7 cells was measured by radioimmunoassay （RIA） ; the effects of kaempferol on the expressions of COX-2 and iNOS protein in RAW264. 7 cells were analyzed by Western blot. Results Kaempferol inhibited the LPS-induced PGE2 and NO synthesis in RAW 264. 7 cells. The protein expression of COX-2 and iNOS in RAW264. 7 cells were also decreased by kaempferol. Conclusions The results show that kaempferol re- duces the augmented biosynthesis of PGE2 and NO by inhibiting the protein expression of COX-2 and iNOS in RAW264.7 cells. The finding may partly explain the anti-inflammatory effect of kaempferol.

关 键 词：山萘酚 环氧合酶-2 诱导型一氧化氮合酶 前列腺素E2 一氧化氮 

分 类 号：R965[医药卫生—药理学]