光质对灵芝菌丝体生长及三萜酸量影响的研究  

Effect of light quality on growth and triterpenic acid content of Ganoderma lucidum mycelium

作　　者：梅锡玲[1] 陈若芸[2] 李保明[2] 陈向东[1] 赵洲[1] 兰进[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《中草药》2013年第24期3546-3550,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81173660)

摘　　要：目的研究光质对灵芝菌丝体生长及三萜酸量和组分的影响。方法采用新型LED冷光源设置不同光质处理,动态观测灵芝菌丝体生长、总三萜酸量及三萜酸组分的变化情况。结果不同光质处理灵芝菌丝体形态、生长速度、生物量和总三萜酸量及单体三萜酸在组分、相对量及各组分间的比例都有所变化。蓝光处理菌丝体在整个培养期始终保持稳定增长;红光处理菌丝体前期生长速度及生物量最大,但是中后期生长速度显著下降,生物量增加缓慢,次于蓝光;绿光处理菌丝体前中期生长速度及生物量均最小,然而培养后期增加明显,干物质迅速积累。灵芝三萜酸在菌丝体培养第7天时已大量产生,随培养时间延长种类增多,组分量降低。绿光和蓝光处理对提高灵芝菌丝体三萜酸种类和量均有明显优势。结论光质对灵芝菌丝体生长及三萜酸量和成分均有显著影响,蓝光对促进灵菌丝体生长及三萜酸积累综合优势明显。Objective To study the effect of light quality on the growth as well as triterpenic acid content and component of Ganoderma lucidum mycelium. Methods Light-emitting diode （LED） was used as sources of light, the growth index, content and component of triterpenic acids in G. lucidum mycelium cultured under different LED conditions were investigated along with culture time. Results The growth rate, morphology, biomass, total contents and components of triterpenic acid in G. lucidum mycelium varied under different lights. The mycelium cultured under blue light maintained stable growth during the whole period; Under red light, both growth rate and biomass of mycelium were the highest in early time, subsequently, decreased sharply and grew slowly. Under green light, both growth rate and biomass of mycelium were the lowest in early and medium phases, while increased significantly later, especially dry matter. Triterpenic acids were highly produced on the day 7 of cultivation, while decreased in content and increased in types. Green light and blue light had a distinct advantage of improving the type and content triterpenic acids in G. lucidum. Conclusion Light quality affects the growth as well as the content and component of triterpenic acids in G. lucidum mycelium, and blue light irradiation is the best for the steady growth and triterpenic acids accumulation.

关 键 词：灵芝 光质 菌丝体生长 三萜酸 HPLC 

分 类 号：R282.2[医药卫生—中药学]