牛传染性鼻气管炎的分离鉴定和灭活条件的优化  

Isolation and identification of infectious bovine rhinotracheitis proliferation and inactivated Optimization

作　　者：梁宏儒[1] 高佳滨[1] 李安[1] 陈为宏[1] 乔波[1] 尹辉[1] 胡旭[1] 赵达[1] 姜东君[1] 朱战波[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2013年第6期35-38,42,共5页journal of heilongjiang bayi agricultural university

摘　　要：从疑似牛传染性鼻气管炎病毒感染的牛鼻腔中分离出1株病毒,通过PCR方法和MDBK细胞病变观察对其进行鉴定,确定该分离株为牛传染性鼻气管炎毒株。通过对该毒株的增殖条件和甲醛灭活条件的优化研究,为制备牛传染性鼻气管炎细胞源灭活疫苗提供参考依据。结果表明该毒株接毒18 h后病变细胞达70%开始收毒,病毒液在0.2%的甲醛37℃灭活36 h灭活效果最好。A virus isolated from bovine nasal swab suspected infectious bovine rhinotracheitis virus infection by PCR and the MDBK cell lesions observed to identify it. The isolates strains of infectious bovine rhinotracheitis. Optimization of the strain proliferation conditions and formaldehyde inactivated conditions,to provide a reference for the preparation of infectious bovine rhinotracheitis cell-derived inactivated vaccine. The results show that the strain inoculation 18 h diseased cells to fight 70% began to collect the drugs,the virus was inactivated in 0.2% formaldehyde 37℃ for 36 h inactivated the best.

关 键 词：牛传染性鼻气管炎病毒 PCR 分离鉴定 甲醛灭活 

分 类 号：S855.3[农业科学—临床兽医学]