HPLC法测定复方苦木消炎片中穿心莲内酯溶出度  

Dissolution Determination of Andrographolide in Compound Kumu Xiaoyan Tablets by HPLC

作　　者：陈刚[1] 李晓凤[1] 苏文俏 刘艺柯 方媛[1,2] 陈祖辉[1] 马宁[1] 

机构地区：[1]长沙医学院,长沙410219 [2]山西大学

出　　处：《中国药师》2013年第12期1799-1802,共4页China Pharmacist

基　　金：长沙医学院2012年度大学生研究性学习和创新性试验计划项目(编号:长医教[2012]21号);2011年度湖南省大学生研究性学习和创新性试验研究计划项目(编号:湘教通[2011]272号)

摘　　要：目的:建立复方苦木消炎片中穿心莲内酯溶出度的测定方法,并对市售产品进行溶出度的测定。方法:采用高效液相色谱法测定穿心莲内酯的溶出度。用500 ml 0.2%十二烷基硫酸钠为溶出介质,使用转篮法在100 r·min^(-1)的转速下进行溶出度的试验,于45 min取样测定。结果:穿心莲内酯的浓度在0.37-3.70μg·ml^(-1)范围内呈良好的线性关系(r=0.999 8),回收率为99.4%,RSD为0.22%(n=6)。在45 min取样测定,复方苦木消炎片中穿心莲内酯的溶出度不低于标示量的80%。结论:本方法简便,结果准确可靠,可用于复方苦木消炎片中穿心莲内酯的溶出度的测定。Objective：To establish a method for the dissolution determination of andrographolide in compound Kumu Xiaoyan tablets and determine the dissolution of a marketed product.Method：Andrographolide was detected by an HPLC method.0.2%Sodium lauryl sulfate（500ml） was used as the dissolution medium.A suspended basket method with a stirring rate of 100 r ？ min-1 was used, and the sampling time was 45min.Result：The dissolution of andrographolide showed good linearity within the range of 0.37-3.70 μg ？ ml-1（r = 0.999 8）.The recovery was 99.4%（RSD = 0.22%,n = 6）.In the sampling time,the dissolution of andrographolide was not below 80%of the labeled contenl.Conclusion：The method is simple with great stability and accuracy,which can be used in the dissolution determination of andrographolide in compound Kumu Xiaoyan tablets.

关 键 词：高效液相色谱法 复方苦木消炎片 穿心莲内酯 溶出度 

分 类 号：R917[医药卫生—药物分析学]