A novel real-time cell electronic analysis technology for the authentication and quality control of natural medicines  

作　　者：Guo-Jun Yan Xin Mei Jin Yang Jenny Zhu Jie-Ying Wu Jin-Huo Pan 

机构地区：[1]College of Pharmacy,Nanjing University of Chinese Medicine [2]ACEA Biosciences,Inc. [3]ACEA BIO (Hangzhou) Co.,Ltd.,Hangzhou High Throughput Drug Screening Center

出　　处：《Chinese Chemical Letters》2013年第12期1140-1144,共5页中国化学快报（英文版）

基　　金：financially supported by a Cultivated Project from Nanjing university of Chinese medicine (No. 10XPY01);the National Natural Science Foundation of China (No. 81202922);a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, PAPD (No. 2011ZYX3-004)

摘　　要：The study was designed to explore the potential applications of the real-time cell electronic analysis technology in the quality evaluation of natural medicines. The natural medicinal Flos Carthami was discussed as a methodological example and the specific time/dose-dependent cell response profiles （TCRPs） were produced by the real-time cell electronic analysis technology. The similarity and bioactivity were obtained by analyzing all TCRPs. Meanwhile, an HPLC method according to the Chinese Pharmacopeia （edition 2010） was used to evaluate the quality of Flos Carthami. The correlation was obtained by comparing the results produced by the two different approaches. By analyzing the data, five different samples ofFlos Carthami can produce remarkably similar TCRPs. The quality ofFlos Carthami was evaluated by both the HPLC and the TCRPs analysis-based approaches and similar results were obtained. The results suggest that the same natural medicine from different locations could produce similar TCRPs. By analyzing the TCRPs, the bioactivity and quality evaluation of natural medicines can be obtained. This technology is a physiologically relevant approach for the quality evaluation of natural medicines. The ultimate aim of our study is to establish a new standard for quality evaluation.The study was designed to explore the potential applications of the real-time cell electronic analysis technology in the quality evaluation of natural medicines. The natural medicinal Flos Carthami was discussed as a methodological example and the specific time/dose-dependent cell response profiles （TCRPs） were produced by the real-time cell electronic analysis technology. The similarity and bioactivity were obtained by analyzing all TCRPs. Meanwhile, an HPLC method according to the Chinese Pharmacopeia （edition 2010） was used to evaluate the quality of Flos Carthami. The correlation was obtained by comparing the results produced by the two different approaches. By analyzing the data, five different samples ofFlos Carthami can produce remarkably similar TCRPs. The quality ofFlos Carthami was evaluated by both the HPLC and the TCRPs analysis-based approaches and similar results were obtained. The results suggest that the same natural medicine from different locations could produce similar TCRPs. By analyzing the TCRPs, the bioactivity and quality evaluation of natural medicines can be obtained. This technology is a physiologically relevant approach for the quality evaluation of natural medicines. The ultimate aim of our study is to establish a new standard for quality evaluation.

关 键 词：Time]dose-dependent cell response profiles （TCRPs） Quality standard Bioactivity Physiologically relevant approach Flos Carthami 

分 类 号：TQ460.1[化学工程—制药化工]