慢性氟中毒大鼠睾丸组织中钙调神经磷酸酶和活化T细胞核因子1的表达  

Expression of calcineurin and nuclear factor of activated T cells 1 in testis of rats with chronic fluorosis

作　　者：邓超男[1] 于燕妮[1] 谢莹[1] 赵丽娜[1] 

机构地区：[1]贵阳医学院病理学教研室,550004

出　　处：《中华预防医学杂志》2013年第12期1142-1147,共6页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金（81260419）;国家科技部国际合作项目（2010DFB30530）;贵州省科技厅社会发展攻关项目（黔科合SY[2011]3006号）

摘　　要：目的 探讨钙调神经磷酸酶（CaN）和活化T细胞核因子1（NFATc1）在慢性氟中毒大鼠睾丸损伤机制中的意义.方法 18只6周龄清洁级雄性SD大鼠按体重用随机数字表法分为3组（每组6只）,对照组饮用自来水（含氟量＜1 mg/L）,低氟组和高氟组分别饮用含5、50 mg/L氟化钠的自来水.饲养8个月后,观察氟斑牙发生情况,氟离子选择电极法检测尿氟含量情况;称量各组大鼠体重及睾丸质量;光镜观察各组大鼠睾丸组织形态学变化;免疫组化和原位杂交检测睾丸组织CaN和NFATc1的蛋白及mRNA表达情况.结果 大鼠氟斑牙检出只数为对照组0只,低氟组4只（4/6）,高氟组5只（5/6）,差异有统计学意义（x2=10.60,P＜0.05）.在对照、低氟组、高氟组尿氟含量依次升高,分别为（1.26 ±0.17）、（2.06±0.64）、（7.69±1.96） mg/L（F=36.57,P＜0.05）;体重分别为（629.00±16.00）、（585.17±17.27）、（560.50±16.07）g,差异有统计学意义（F=26.67,P＜0.05）;睾丸质量分别为（2.58 ±0.17）、（2.43±0.31）、（2.35 ±0.38）g,差异无统计学意义（F=0.91,P＞0.05）.与对照组比较,各染氟组大鼠各级生精小管结构出现不同程度破坏,高氟组破坏程度最大.对照组、低氟组、高氟组睾丸组织中CaN蛋白表达灰度值依次增加,分别为59.10±5.62、77.93±4.16、101.69 ±6.31 （F=74.18,P＜0.05）;NFATc1蛋白表达灰度值分别为76.11 ±4.41、93.42±3.85、120.42±9.31,随染氟增加而逐渐增加（F =92.4,P＜0.05）.CaN的mRNA表达量分别为58.76±7.70、82.01±6.88、99.47±8.33（F=42.65,P＜0.05）,NFATc1分别为59.39±4.74、90.02±5.37、121.15±7.69（F=155.47,P＜0.05）.CaN和NFATc1蛋白及mRNA表达水平呈正相关（r分别为0.899、0.908）.结论 CaN依赖的信号通路表达变化可能参与到慢性氟中毒大鼠睾丸组织的损伤机制中.Objective To discuss the significance of calcineurin （CaN） and nuclear factor of active T cells 1 （NFATcl） in the damage mechanism of the testis of rats with chronic /luorosis. Methods Eighteen clear class SD male rats, aging 6 week-old, were randomly divided into 3 groups, 6 rats in each. The rats of control group were fed with tap water （ NaF 〈 1 mg/L） and the experimental rats were exposed to NaF （lower group： 5 rag/L, higher group： 50 rag/L） to established the chronic flunrosis model. After 8 months, we observed the occurance of dental fluorosis among rats in different groups, and the contents of urine fluoride were detected by fluorine ion selective electrode method. The body of the rats were weighted as well as their testis. The testis tissues were stained with henlatoxylin-eosin and observed under light microscope to find the morphological changes. The expression of CaN and NFATcl＇s protein and mRNA in testis were detected by Imnmnoeytochemistry （IHC） and In-situ hybridization （ISH）. Results The number of rats which was found dental fluorosis were separately 0, 4 and 5 in control group, low dose group and high dose group （ X2 = 10. 60, P 〈 0. 05 ）. The contents of urine fluoride were gradually increased in control group, low group and high group, which were （ 1.26 ±0. 17 ）, （ 2. 06 ±0. 64 ） and （ 7.69 ±1.96 ） mg/L, respectively （ F = 36.57, P 〈 0. 05 ） . The body weight were significantly different in all three groups （629.00 ± 16.00）, （585.17 ± 17.27 ）, （ 560. 50 ± 16. 07 ） g, F = 26. 67, P 〈 0. 05 ） and the testis weight were without statistical difference （ （ 2. 58± 0. 17 ）, （ 2.43 ± 0. 31 ） , （2. 35 ± 0. 38 ） g, F = 0. 91, P 〉 0. 05 ）. Compared with the control group, the testicular structures were damaged in the experimental groups and especially significant in high dose group. The expression of CaN （59. 10 ±5.62, 77.93 ±4. 16, 101.69 ±6. 31, F=74. 18,P〈0. 05） and NFATcl＇s （76. 11 ±4. 41, 9

关 键 词：氟化物 睾丸 钙调神经磷酸酶 活化T细胞核因子 

分 类 号：R595.1[医药卫生—内科学]