乙肝转阴散微生物限度检查方法验证研究  

Study on the Method of Microbial Limit Test for Yiganzhuanyin powders

作　　者：吕建伟[1] 唐勇琛[1] 潘革卉[1] 

机构地区：[1]广西壮族自治区柳州市中医院药学部,545001

出　　处：《检验医学与临床》2013年第24期3310-3311,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨乙肝转阴散的微生物限度分析和检查方法的有效性。方法选择5种菌为阳性对照,采用平皿计数法,采用回收率试验对细菌、丝状真菌及酵母菌计数方法的回收率进行验证;采用相同的实验条件,观察大肠埃希菌、大肠菌群、沙门菌在试验组、阳性对照组和阴性对照组的检出情况验证控制菌检查方法。结果经方法学验证试验得出,乙肝转阴散有抑菌作用,其微生物限度检查计数方法采用培养基稀释法,控制菌检查采用常规法。结论验证后的微生物限度检查方法简便可行、准确无误,适合于乙肝转阴散的微生物限度检查。Objective To establish an examination method for microbial limit determination of Yiganzhuanyin powders .Methods Plate counting method was used .The method of counting bacteria and mould was validated by the recovery rates with 5 control trains .The method of checking control bacteria was validated by observing cultivation of Escherichia coli、coliform group and salmonella in the test group、positive control group and negative control group under the same environment .Results The results of validation test showed that Yiganzhuanyin powders had antibac-terial activity .The culture media dilution method was used as the counting methods for the bacteria detection can be carried out by the conwentional examination method .Conclusion The method was simple ,feasible ,reliable and could be used for the examination of microbacteria limit .

关 键 词：乙肝转阴散 微生物限度检查 方法学验证 

分 类 号：R927.33[医药卫生—药学]