清络通痹颗粒通过MAPKs信号通路干预共培养诱生的破骨样细胞的分化  

Influence of MAPK pathways on co-cultured osteoclasts differentiation and effects of Qingluotongbi granule on MAPK expressions

作　　者：周玲玲[1] 柳璋璞[1] 周聪[1] 陈晨[1] 刘天阳[1] 张莹[1] 周学平[1] 

机构地区：[1]南京中医药大学,江苏省中药药效与安全性评价重点实验室,南京210029

出　　处：《中国免疫学杂志》2013年第12期1235-1239,共5页Chinese Journal of Immunology

基　　金：国家自然基金(81072749);江苏省六大人才高峰课题(2007)资助

摘　　要：目的:研究MAPKs通路对共培养诱生的破骨样细胞分化和活性的调控及清络通痹颗粒对MAPKs通路的影响。方法:分离佐剂性关节炎大鼠外周血单核细胞和滑膜成纤维细胞,共培养诱生破骨样细胞;共培养体系加入MAPK抑制剂观察破骨细胞的增殖、TRAP活性和骨吸收功能的变化;加入清络通痹颗粒含药血清,实时定量PCR观察其对破骨样细胞ERK、JNK和p38表达的影响,Western blot法观察其对破骨样细胞磷酸化ERK、JNK和p38表达的影响。结果:加入MAPK抑制剂U0126、SP600125、SB203580后,共培养诱生的破骨样细胞增殖能力、TRAP活性和骨吸收面积均受到明显抑制;清络通痹颗粒3.6、7.2、14.4 g/kg给药的大鼠含药血清可不同程度抑制破骨样细胞ERK、JNK和p38的表达,可明显抑制破骨样细胞磷酸化ERK、JNK和p38的表达。结论:MAPKs通路在破骨细胞分化过程中起着重要的作用,清络通痹颗粒可抑制MAPKs通路的活化而进一步抑制破骨细胞的分化、增殖和活性。Objective：To study how MAPK pathways influence the differentiation of co-cultured osteoclasts and the effects of Qingluotongbi granule （QLT） on the expressions of MAPK proteins. Methods： The monocytes of peripheral blood and synovial fibro- blasts of adjuvant-induced arthritic rats were separated and co-cultured to induce osteo-like cells. MAPK inhibitors U0126, SP600125 and SB203580 were added into the co-culture medium and the proliferation, TRAP activity and bone resorption of osteoclasts were ob- served. QLT-containing sera were obtained and added to the co-cultured system, and the expression of ERK, JNK and p38 were detected by Reahime PCR. The expression of p-ERK, p-JNK and p-p38 were detected by Western blot simultaneously. Results： U0126, SP600125 and SB203580 could inhibit the proliferation, TRAP activity and bone resorption of co-cultured osteoclasts. QLT- containing sera could inhibit the expression of ERK, JNK and p38 in osteoelasts. QLT-containing sera could also inhibit the expression of p-ERK, p-JNK and p-p38. Conclusion： MAPK pathways participated in the differentiation and activity of osteoclasts. Qingluotongbi granule could inhibit the differentiation and activity of osteoclasts by inhibiting the activity of MAPKs pathways.

关 键 词：清络通痹颗粒 类风湿关节炎 破骨细胞 MAPKS 

分 类 号：R285.5[医药卫生—中药学]