干扰素-α阻断地塞米松对神经细胞p11表达的上调作用  

Interferon-alpha blocks dexamethason-induced upregulation of p11 expression in SH-Sy5y cells

作　　者：张莉莉[1] 郭继强[1] 张文[1] 刘威[1] 张志永[1] 栾好江[1] 王辉[1] 

机构地区：[1]新乡医学院免疫学研究中心,新乡453003

出　　处：《中国免疫学杂志》2013年第12期1254-1257,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.81241095);河南省教育厅科学技术研究重点项目(12B320018)

摘　　要：目的:探讨干扰素-α联合地塞米松对神经细胞p11表达的影响。方法:神经细胞经过10-7mol/L地塞米松或和1 000 U/ml IFN-α处理后,利用RT-PCR检测各组p11 mRNA的表达水平;Western blot法检测p11蛋白的表达水平;免疫荧光检测各组p11蛋白的表达水平。结果:Real-time PCR和Western blot结果提示1 000 U/ml IFN-α刺激组p11的表达降低,10-7mol/L地塞米松刺激组p11的表达升高;IFN-α和地塞米松联合刺激组与对照组相比无显著差异。免疫荧光结果也显示IFN-α刺激组可抑制p11蛋白的表达,p11在IFN-α联合地塞米松组中的表达低于地塞米松组。结论:IFN-α可以下调神经细胞p11表达;也可以阻断地塞米松对p11表达的上调作用。Objective：To investigate the effects of interferon-α combined with dexamethasone on pl 1 expression in nerve cell line. Methods： After treatment with 10-7 mol/L dexamethasone （DEX） or/and 1 000 U/ml interferon-α in nerve ceils, the expres- sions of pl 1 mRNA and protein in each group were determined using RT-PCR and Western blot respectively. Immunofluorescence was also performed to detect pl 1 expression. Results： RT-PCR and Western blot results showed that pl I expression was decreased in 1 000 U/ml interferon-α treatment group and increased in DEX treatment group. There was no significant difference in pl 1 expression be- tween combination group and control group. Immunofluorescence showed that pl 1 expression was inhibited in interferon-α treatment group and its expression in the combination group was decreased compared with DEX treatment group. Conclusion： Interferon-α could inhibit the expression of pl 1 and block dexamethasone-induced increase of pl 1 expression in nerve cells.

关 键 词：地塞米松 干扰素-Α P11 

分 类 号：R392.12[医药卫生—免疫学]