胎膜早破患者HBD-2、NF-κB p56的表达及其相关性  

Expression of HBD-2,NF-κB p56 and theirs correlation in patients with prema-ture rupture of membrane

作　　者：颜露春[1] 严兆华[1] 庞天云[1] 李小林[1] 曾三梅[1] 

机构地区：[1]广东医学院附属医院妇产科,湛江524001

出　　处：《中国免疫学杂志》2013年第12期1281-1284,1287,共5页Chinese Journal of Immunology

基　　金：广东省医学科研基金项目(No.B2011247);广东医学院附属医院青年基金项目(No.QX1119)

摘　　要：目的：探讨孕妇血清、胎盘、胎膜中人β-防御素-2（Human beta-defensins-2,HBD-2）、核因子-κB（nuclear factor-κB p56,NF-κB p56）水平相关性,以及与胎膜早破（Premature Rupture of Membrane,PROM）的关系,初步探讨 HBD-2、NF-κB p56在PROM的作用,为临床诊治PROM提供新的思路.方法：PROM组：妊娠37～42周35例,34～36＋6周35例,28～33＋6周25例;与胎膜早破组孕周、例数相对应的95例非胎膜早破孕妇作为对照组.根据胎膜病理诊断结果,在PROM组分为绒毛膜羊膜炎组（histological chorioamnionitis,HCA）组与非HCA组,采用ELISA两步法测定外周血HBD-2、NF-κB p56,SYBR GreenⅠ实时荧光定量RT-PCR检测胎盘,胎膜HBD-2、NF-κB p56的mRNA表达,比较胎膜早破组与对照组,HCA组与非HCA组在不同孕周孕妇血清,胎盘,胎膜HBD-2、NF-κB p56测定结果的差异,并进行相关性分析.结果：（1）妊娠37～42周：PROM组孕妇入院血清,分娩结束血清,胎盘,胎膜HBD-2、NF-κB p56 的表达水平与对照组比较差异无统计学意义（P均〉0.05）;（2）妊娠34～36周＋6 和妊娠28～33周＋6：PROM组孕妇入院血清,分娩结束血清,胎盘,胎膜HBD-2、NF-κB p56的表达显著高于对照组（P均〈0.05）;PROM组分娩结束血清HBD-2、NF-κB p56的表达高于入院血清,差异有统计学意义（P均〈0.05）;（3）HCA组孕妇分娩结束血清,胎盘,胎膜中HBD-2、NF-κB p56的表达与非HCA组比较,差异有统计学意义（P均〈0.05）,而入院血清HBD-2、NF-κB p56表达差异无统计学意义（P均〉0.05）;（4）妊娠37～42周：入院血清,分娩结束血清,胎盘,胎膜表达HBD-2、NF-κB水平无相关关系（P〉0.05）,同一组织中HBD-2与NF-κB p56水平无相关关系（P〉0.05）;（5） 妊娠34～36周＋6和妊娠28～33周＋6：入院血清HBD-2,分娩结束血清HBD-2呈正相关r=0.82（P＜0.01）,入院血清HBD-2,分娩结束血清HBD-2均与胎盘HBD-2水平呈正相关关系r=0.66、0.65（P＜0.0Objective：To determine the relationships between maternal serum, embryolemma, placenta of human beta-de- fensins-2, （HBD-2） ,nuclear factor-κB p56, （NF-κB p56） in pregnant women with premature rupture of membrane （PROM）and with- out PROM. Methods： Ninety five women with PROM were enrolled in the study. And divided into 37 -42 weeks,34 -36.6 weeks, 28 - 33 ＋6 weeks according to gestational age. The same sample without PROM pregnancies matching the same gestational ages were re- cruited as control group. According to the results of pathological diagnosis of membranes, pregnant women with PROM divided into his- tological chorioamnionitis, （HCA） and non-HCA. We examined the messenger RNA （mRNA） of HBD-2 and NF-κB p56 in placenta and fetal membrane real-time reverse transcription polymerase chain reactions by dsDNA-binding dyes of SYBR Green, the protein of their in maternal serum using ELISA. Results： （ 1 ） For 37 - 42 weeks pregnant women ： there had no difference between two groups （ P 〉 0. 05 ） ; （2） For 34 - 36 ＋ 6 weeks and 28-33 ＋ 6 weeks pregnant women ： in hospital serum, serum end of birth, the placenta, fe- tal membrane, the level of HBD-2 and NF-κB p56in PROM group are significantly higher than control group （P 〈 0.05 ） ; serum end of birth is higher than hospital serum in PROM （ P 〈 0. 05 ） ; （ 3 ） In hospital serum, serum end of birth, the placenta, fetal membrane, the level of HBD-2 and NF-κB p56 in HCA group are significantly, higher than non-HCA group （ P 〈 0. 05 ）, but not in hospital serum （P 〉0.05） ; （4） For 37 -42 weeks pregnant women： there had no correlation between hospital serum, serum end of birth, the pla-centa, fetal membrane of the expression HBD-2 and NF-κB p56 （P 〉 0. 05 ） , there had no correlation between HBD-2 and NF-κB p56 in the same organization （ P 〉 0. 05 ） ; （5） For 34 - 36 ＋ 6 weeks and 28 - 33 -＋6 weeks pregnant women ： there were positive correla- tions between hos

关 键 词：胎膜早破 绒毛膜羊膜炎 人Β-防御素-2 核因子-ΚB p56 

分 类 号：R714.7[医药卫生—妇产科学]