水稻苗期耐冷性相关QTL qCTS3-1的鉴定和分子定位  

Identification and mapping of a major quantitative trait locus (QTL) qCTS3-1 controlling cold tolerance in rice seedling

作　　者：周勇[1] 王中德[1] 陶亚军[1] 缪军[1] 高云[1] 魏伟[1] 梁国华[1] 

机构地区：[1]扬州大学,教育部植物功能基因组学重点实验室/江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《江苏农业学报》2013年第6期1181-1186,共6页Jiangsu Journal of Agricultural Sciences

基　　金：高等学校博士学科点专项科研基金项目(20103250110004;20113250120001);国家自然科学基金项目(31071068;31100863)

摘　　要：低温会影响水稻正常的生长和发育,增强耐冷性已成为水稻育种的主要目标之一.为了鉴定和定位水稻耐冷性相关位点,本研究以广陆矮4号（受体）与日本晴（供体）杂交获得的染色体片段代换系群体中的1个低温敏感株系C32022为材料,测定低温处理后植株存活率,采用代换作图法在水稻第3染色体上定位一个控制水稻苗期耐冷性的QTL,命名为qCTS3-1.利用C32022和广陆矮4号衍生的F2分离群体进行遗传分析,结果显示,该群体中叶片表现正常绿色与叶片表现出黄色、萎蔫单株比例符合1∶3分离比,表明耐冷性受单个主效QTL控制.来自日本晴的等位基因能够增加植株对低温的敏感性.利用该群体中86个隐性单株将qCTS3-1初步定位在分子标记WZD-S3-7～WZD-S3-11,物理距离约为550 kb.Low-temperature stress, affecting the normal growth and development, is a serious abiotic stress in rice （ Oryza sativa L. ）. Cold tolerance has long been an important objective in rice breeding. To characterize and map QTLs asso- ciated with rice cold tolerance, a low-temperature sensitive line C32022 from a set of chromosome segment substitution lines derived from a cross between Guangluai 4 as the recurrent parent and Nipponbare as the donor was characterized. Survival rate of rice seedling after cold treatment was determined. A major QTL, named qCTS3-1, was mapped on chromosome 3 by substitution mapping. In a F2 segregating population consisting of 401 plants derived from C32022 and Guangluai 4, the fre- quency distribution of individuals with normal green leaves and individuals with yellow leaves was shown to be an expected 1 ： 3 ratio, suggesting that the cold tolerance was controlled by single Mendelian factor in this population. Segregation analy- sis also indicated that the Nipponbare allele increased the sensitivity to low temperature. The qCTS3-1 locus was further narrowed down to approximately 550 kb DNA fragment defined by newly developed STS markers from WZD-S3-7 to WZD- $3-1! using 86 recessive individuals.

关 键 词：水稻 耐冷性 数量性状位点 分子定位 

分 类 号：S511.034[农业科学—作物学]