狼尾蕨体细胞耐盐突变体筛选技术  

Technique for salt-tolerant somatic mutant screening in Davallia bullata

作　　者：叶晓青[1] 佘建明[1] 贾新平[1] 梁丽建[1] 尤仙 

机构地区：[1]江苏省农业科学院农业生物技术研究所,江苏省农业生物学重点实验室,江苏南京210014

出　　处：《江苏农业学报》2013年第6期1266-1270,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省科技厅科技支撑项目(BE2010339);江苏省农业科技自主创新基金项目[CX(13)2044]

摘　　要：以狼尾蕨无菌苗叶片为外植体,探讨不同浓度的氯化钠(NaCl)对叶片诱导不定芽的影响。直接筛选法结果显示:当NaCl浓度为0(对照)时,叶片不定芽诱导率为80.2%;0.4%、0.5%、0.6%和0.7%NaCl处理下,不定芽诱导率分别降至28.4%、20.6%、15.9%和7.8%;0.8%NaCl处理下,叶片完全丧失不定芽的诱导能力。SSR分子标记检测结果显示,248对引物组合中有9对引物在狼尾蕨野生型和耐盐筛选植株间PCR扩增存在多态,其中引物WXE-56和WXE-204可作为耐盐突变体SSR分子标记筛选的适宜引物。The leaves of sterile plantlet of Davallia bullata were used as materials to study the effect of different con- centrations of sodium chloride （NaC1） on induction of the adventitious bud. The induced rate of adventitious bud without NaC1 treatment（CK） was 80. 2%, and the induced rate treated by 0.4%, 0. 5%, 0. 6%, 0. 7% and 0. 8% NaC1 droped to 28.4%, 20. 6%, 15.9%, 7.8% and 0, respectively. Among 248 pairs of specific simple sequence repeat（SSR） prim- ers, nine pairs were associated with the salt tolerance of somatic mutants, among which, WXE-56 and WXE-204, were suitable for identification of the salt-tolerant mutant.

关 键 词：狼尾蕨 叶片 不定芽 体细胞突变体 耐盐 SSR标记 

分 类 号：S682.35[农业科学—观赏园艺]