蒙古绵羊输卵管组织中β-防御素表达的原位杂交技术检测  

Expression of β-defensins in the fallopian tubes of Mongolian sheep detected by in situ hybridization

作　　者：锡林高娃[1,2] 任洪宝[1] 曹贵方[3] 

机构地区：[1]内蒙古民族大学生命科学学院,内蒙古通辽028400 [2]内蒙古民族大学乳源性致病菌研究所,内蒙古通辽028400 [3]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《江苏农业学报》2013年第6期1377-1379,共3页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30460093);内蒙古民族大学科学研究项目(MDX2008022)

摘　　要：β-防御素(β-defensin)是一类大量存在于哺乳动物上皮组织内的内源性抗微生物肽,它们在非特异性免疫反应中占重要地位。为阐明蒙古绵羊β-防御素(Sheep beta-defensin,sBD-1)在输卵管组织内的表达定位,以扩增的sBD-1 cDNA作为探针,并采用核酸原位杂交技术进行检测。结果表明:sBD-1 mRNA在蒙古绵羊输卵管黏膜层上皮细胞游离面的胞质内表达,固有层、肌层、结缔组织中无表达,符合β-防御素主要表达于黏膜表面细胞的一般规律。β-defensin, a kind of endogenous antimicrobial peptides, distributed in epithelial tissue in the kingdoms of Mammalian, plays important roles in the non-specific immune responses. To determine the localization and expression of Mongolian sheep β-defensin （ Sheep beta-defensin, sBD-1 ） in the oviductal issues, in situ hybridization technology was em- ployed, using the cloned sBD-1 cDNA as a probe. The results reveal that sBD-1 mRNA was expressed in the cytoplasm on epithelial cells free surface of Monglian oviductal tissues, and no expression was found in lamina propria, muscle and con- nective tissue, which was in accordance with the ordinary rule of β-defensin＇ s major expression in mucosal surface of cells.

关 键 词：Β-防御素 组织表达 原位杂交 

分 类 号：S826.2[农业科学—畜牧学]