DNA条形码序列对不同品种美国山核桃的鉴定  

Identification of pecan varieties by DNA barcoding

作　　者：陈亚辉[1] 朱海军[1] 生静雅[1] 张普娟[1] 鲍瑾[1] 刘广勤[1] 

机构地区：[1]江苏省农业科学院园艺研究所,江苏南京210095

出　　处：《江苏农业学报》2013年第6期1445-1450,共6页Jiangsu Journal of Agricultural Sciences

基　　金：2013年江苏省林业三新工程项目[lysx(2013)06];2011年国家林业公益性行业科研专项基金项目(201204404);2012年中央财政林业科技推广示范基金项目[(2012)TK28]

摘　　要：选择ITS、ITS2、psbA-trnH、matK 4条序列进行评价,以扩增及测序成功率、变异位点数和k-2-p距离等指标评价各序列的鉴定能力。此外,基于MEGA5分析不同品种美国山核桃序列种间K2P遗传距离并构建NJ树。结果显示:不同品种美国山核桃4条序列的扩增及测序成功率扩增均为100%。不同品种的ITS2和psbA-trnH序列一致性为100%,而ITS和matK序列的一致性分别为99.32%和99.95%,两者均存在较多的变异位点,可以有效地区分美国山核桃不同品种。因此,ITS和matK序列可以作为鉴定美国山核桃不同品种的优势条形码组合。The ITS2, psbA-trnH, ITS and matK sequences were used for identification of pecan varieties. The PCR amplification and sequencing efficiency, variation sites and k-2-p distance were employed to evaluate their capa- bilities of identification. In addition, K2P distances were calculated and NJ Tree was constructed. The result showed that the amplification and sequencing efficiencies of all pecan varieties were 100.0%. The identity of ITS2 sequence in pecan varieties were 100.0% , and so did psbA-trnH sequence. The similarities of ITS and matK in pecan varieties were 99.32% and 99.95% , respectively. Many variation sites in ITS and matK could help to identify pecan varieties efficiently.

关 键 词：DNA条形码 美国山核桃 品种鉴定 

分 类 号：S664.1[农业科学—果树学]