应用高效液相色谱法对饲料中五种抗生素的同时检测  

Simultaneous determination of many antibiotics in feedstuff by High Performance Liquid Chromatography

作　　者：李丽[1] 王金晶[1] 陈立江[1] 刘宇[1] 王菁[1] 焉喜臣[1] 

机构地区：[1]辽宁大学药学院,辽宁沈阳110036

出　　处：《现代畜牧兽医》2013年第12期44-48,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：沈阳市科技计划项目"动物饲料中多种抗生素检测的技术服务"(F11-255-4-00)

摘　　要：本试验建立了同时测定饲料中5种抗生素含量的高效液相色谱法。试验采用梯度洗脱，使金霉素、氯霉素、红霉素、罗红霉素、青霉素得到较好的分离；柱温30℃，流速为1．0mL／min，进样量20μL，检测波长210nm。结果显示，金霉素、氯霉素、青霉素标准曲线线性范围为0．5～40μg／mL，红霉素、罗红霉素标准曲线线性范围为0．5～80μg／mL，相关系数r≥0．9997。饲料中5种抗生素的加样回收率为95．86％-98．72％，RSD〈2％。本试验建立的梯度洗脱方法简便快速，准确度和精密度较高，可以满足饲料中CTC、CPA、ERY、Rox、PG的检测。In our study, we established an efficient method for simultaneous detec- tion of 5 antibiotics in feed by high-performance liquid chromatography was estab- lished. Chlortetracycline（CTC）, chloramphenicol（CAP）, erythromycin（ERY）, roxithromy- cin（ROX） and benzylpenicillin potassium（PG） were separated thoroughly by gradient elution under the conditions of column temperature at 30 ℃, flow rate of 1.0 mL/ min, and with the injection volume of 20 μ L. The signal was detected at 210 nm. The results showed that a linear range was found between peak height and concentration in the range of 0.5-40 μ g/mL for CTC, CAP and PG, and 0.5-80 μ g/mL for ERY and ROX. The linear regression coefficients were not less than 0.999 7. The recovery rates of the five antibiotics were from 95.86% to 98.72%, and RSD was less than 2%. High-performance liquid chromatography is simple, rapid and has high accuracy and precision, which meets the detection of CTC, CPA, ERY, ROX and PG in feedstuff.

关 键 词：高效液相色谱法 金霉素 氯霉素 红霉素 罗红霉素 青霉素 

分 类 号：S816.73[农业科学—饲料科学]