不同产地五味子的木脂素类成分的含量测定  

Content Ditermination on Lignans in Schisandra Chinensis from Different Origin

作　　者：周悌强[1] 冯素香[1] 李晓玉[1] 白燕[1] 李建生[1] 李娟[1] 徐鹏[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《医药导报》2013年第12期1624-1627,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81130062)

摘　　要：目的 用反相高效液相色谱（RP-HPLC）法同时测定五味子中五味子醇甲、五味子酯甲、五味子甲素及五味子乙素的含量,对不同产地五味子的木脂素类成分进行研究.方法 采用Venusil XBP C18色谱柱（4.6 mm×250 mm,5 μm）,流动相为甲醇（A）-水（B）,梯度洗脱（0～25 min,A 为72%;26～50 min,A 为77%）,流速1 mL·min-1,柱温25 ℃,检测波长250 nm.结果 五味子醇甲、五味子酯甲、五味子甲素及五味子乙素分别在1.27～10.17 μg（r＝0.999 6）,0.27～2.13 μg（r＝0.999 0）,0.32～2.52 μg（r＝0.999 5）,0.89～7.19 μg（r＝0.999 4）范围内呈良好的线性关系;平均回收率分别为100.51%,101.19%,102.05%,99.73%,RSD分别为2.09%,1.83%,1.99%,1.34%.结论 该方法准确、简便、分离好,无干扰,可为完善五味子质量评价提供依据.Objective To establish a method for determining schizandrin, schisantherin A, deoxyschizandrin and γ- schishandrin in Schisandra chinensis by RP-HPLC, and to study lignans composition of Schisandra chinensis from different origins. Methods A Venusil XBP Ci8 column（4.6 mmx250 nun, 5 tam） column was used with gradient elution by methanol （A）-water（B） （0-25 min, 72% A; 26-50min, 77% A） as the mobile phase. The flow rate was 1.0 mE · min-1. The column temperature was 25 ℃. The detection wavelength was set at 250 nm. Results The calibration curves of schizandrin, schisantherin A, deoxyschizandrin and γ-schishandrin were linear within the ranges of 1.27- 10. 17 tag （r = 0. 999 6 ） , 0.27- 2.13 μg（ r= 0. 999 0）, 0.32-2.52 μg（ r= 0. 999 5）, and 0.89-7.19 tag（ r= 0. 999 4） , respectively,. The average recoveries were 100.51% （RSD of 2.09% ）, 101.20% （RSD of 1.83% ）, 102.05% （RSD of 1.99% ）, 99.73% （RSD of 1.34% ）, respectively. Conclusion The method is accurate, simple and selective, providing a basis for improvement of quality evaluation for Schisandra chinensis.

关 键 词：五味子 五味子醇甲 五味子酯甲 五味子甲素 五味子乙素 含量测定 

分 类 号：R282[医药卫生—中药学] R927.2[医药卫生—中医学]