紫外诱变木质素降解菌株的初步研究  

A preliminary study on mutation of lignin degrading fungal strain by ultraviolate irradiation

作　　者：张祥胜[1,2] 曾娟[2] 邹学东[1] 

机构地区：[1]胜达集团江苏双灯纸业有限公司,江苏盐城224341 [2]盐城师范学院生命科学与技术学院,江苏盐城224051

出　　处：《中华纸业》2013年第24期27-30,共4页China Pulp & Paper Industry

基　　金：胜达集团江苏双灯纸业有限公司企业合作课题;江苏省博士聚集计划资助项目

摘　　要：近年来，生物制浆技术因其环保、低成本而成为制浆造纸行业的研究方向之·，白腐菌是降解木质素最有效的微生物。本文以生物制浆用白腐菌菌株z9为研究对象，对筛选培养基进行筛选，并测其对稻草、稻灰中木质素的降解率，并通过紫外诱变获得优良突变体，以期为制浆造纸企业提供优良菌株。结果显示：（1）Z9在愈创木酚平板和溴酚蓝平板培养时现象最明显，可作为诱变后的筛选平板，其中以愈创木酚平板最佳；（2）木质素的降解率分别为22．O％和12．5％，表明z9可用作生物技术辅助制浆的菌种；（5）z9紫外照射较合适的时间为60-80s，此时存活率为30％左右；（4）通过愈创木酚平板筛选并结合测定木质素降解率和相关酶活，获得一个综合性状优良的突变株Z9-9。In recent years, bio-pulping has become a research focus in pulp making and pulp and paper industry, as an environmentally friendly, and low cost technology. White-rot fungus is the most effective microorganism to degrade lignin. In this study, a white-rot fungus strain Z9 was studied. Three kinds of screening medium were employed to evaluate its lignin degrading capacities. Z9 was also submitted to ultraviolate irradiation and the survival rates at different exposure durations were measured and the mutants was screened out to provide mutants for pulping and papermaking enterprises. Experimental results showed that： （1） when Z9 was cultivated on methyl catechol and bromophenol blue plates, clear circles were most notable. Both two kinds of screening plates can be used as screening plates after ultraviolet irradiation and methyl catechol plate is the best for screening plate; （2） the degradation rate of lignin was respectively 22.0% and 12.5%, showing it could be used as the effective fungus species of biotechnology aided pulping; （3） the optimal exposure time of ultraviolet irradiation for Z9 was 60s to 80s, at which the survival rate was about 30%; （4） after ultraviolate irradiation, a mutant Z9-9 with good comprehensive performance was screened out through methyl catechol plate and measurement of lignin degradation rate and relevant enzyme activities.

关 键 词：生物制浆 白腐菌 木质素降解酶 紫外照射 

分 类 号：TS713[轻工技术与工程—制浆造纸工程]