蛇油中脂肪酸成分的GC-MS分析  

Discuss Technology of Sharp-snouted Fu'Domesticate and Breed in Xiang-xi

作　　者：彭德姣[1,2] 陈玲[1,2] 

机构地区：[1]吉首大学生态旅游湖南省重点实验室,湖南张家界427000 [2]吉首大学生态所,乌龙山蛇类研究所,湖南吉首416000

出　　处：《蛇志》2013年第4期367-368,共2页Journal of Snake

基　　金：湖南省科技厅(2010SK303);吉首大学(JDSTLY201110);国家科技型中小企业技术创新基金(12C26214304972)

摘　　要：目的为充分利用蛇副产品资源,增加其附加值,探究其功用。方法取蛇内脏脂肪组织,用微波加热法得蛇油,将其甲酯化后低温保存,采用气相色谱-质谱(GC-MS)技术和标准图谱检索对照,分离和鉴定蛇油的组成成分,用离子流色谱峰面积归一化法计算各脂肪酸成分的相对含量,取峰值较高的15种脂肪酸成分进行分析。结果蛇油中含有大量不饱和脂肪酸,占总脂肪酸含量的52%以上。结论蛇油脂利用价值较高,且含有部分对人体具有十分重要生理功能的高度不饱和脂肪酸,特别是EPA、DHA。Objective To make full use of the snake by-product resources,increase the added value,probe into its function. Methods Getting adipose tissue out of snakes innards and obtaining clear,flaxen,liquid snake oil through heating method of microwave furnace.Refrigeratting the methylesterificated snake oil.Then isolating and identificating the components of snake iol by GS-MS and standard atlas retrieval control methods and calculateing relative contents of different fatty acid components by Anti-anginal chromatographic peak area normalization meth-od. Results The snake oil contain a lot of unsaturated fatty acid which made up over 52%. Conclusion The snake oil is value very high,some highly unsaturated fatty acids are key important for human physiological func-tion,EPA and DHA are outstanding.

关 键 词：乌梢蛇 蛇油 脂肪酸 GC-MS 

分 类 号：S865.32[农业科学—野生动物驯养] O623.61[农业科学—畜牧兽医]