糖、硼、钙对大喇叭杜鹃四合花粉萌发的影响  

Effects of Sucrose,Boron and Calcium on the Tetrad Pollen of Rhododendron excellens In Vitro

作　　者：白天[1] 解玮佳[2,3,4] 李世峰[2,3,4] 关文灵[1] 宋杰[2,3,4] 李树发[2,3,4] 蔡艳飞[2,3,4] 陆琳[2,3,4] 李进昆[2,3,4] 

机构地区：[1]云南农业大学园林园艺学院,昆明650201 [2]云南省农业科学院花卉研究所,昆明650205 [3]云南省花卉育种重点实验室,昆明650205 [4]云南省花卉工程中心,昆明650205

出　　处：《上海交通大学学报（农业科学版）》2013年第6期48-54,60,共8页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：云南省科技计划项目(2011EB110)

摘　　要：以大喇叭杜鹃(Rhododendron excellens)为研究对象,研究了蔗糖、硼酸及CaCl2单因子及正交试验对其四合花粉萌发和花粉管生长的影响。结果表明,蔗糖、硼酸和CaCl2及3因子交互效应对大喇叭杜鹃的四合花粉萌发率、单花粉粒萌发数量及单花粉粒花粉管长度均有显著影响;最适蔗糖、硼酸和CaCl2溶液浓度分别为80g/L、100mg/L、80mg/L;正交试验的最佳配方组合是:80g/L蔗糖+50mg/L H3BO3+40mg/L CaCl2。The effects of sucrose, H3BO3 and CaCl2 single factor and orthogonal test on tetrad pollen germination and pollen tube growth of Rhododendron ecccellens were studied. The results indicated that the sucrose, H3BO3 and CaCl2 had significant effects on the tetrad pollen germination rate, pollen germination quantity and pollen tube average length of tetrad pollen of Rhododendron eacellens. The single suitable concentration of sucrose, H3BO3 and CaCl2 for the tetrad pollen germination rate,single pollen germination quantity and single pollen tube growth of tetrad pollen were 80 g/L,100 mg/L and 80 mg/L, respectively. The optimal germination medium of tetrad pollen germination and tube growth was 80 g/L Sucrose ＋ 50 mg/L H3BO3＋40 mg/L CaCl2.

关 键 词：大喇叭杜鹃 蔗糖 硼酸 CACL2 四合花粉萌发 

分 类 号：Q945.34[生物学—植物学] S685.21[农业科学—观赏园艺]