meso-四-[4-(Boc-苏氨酸)氨基苯基]卟啉荧光探针对L-色氨酸的痕量测定  

Determination of Trace Amounts of L-tryptophan Using Tetra- [(4-Bocthreonine)-aminophenyl]Porphyrin as Fluorescence Spectral Probe

作　　者：蔡燕燕[1] 谭卫红[1] 马艳[1] 沈娟章[1] 魏琴[2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,江苏南京210042 [2]济南大学化学化工学院,山东济南250022

出　　处：《分析测试技术与仪器》2013年第4期193-198,共6页Analysis and Testing Technology and Instruments

基　　金：国家自然科学基金(20577016);山东省自然科学基金(Y2008B44)

摘　　要：利用荧光光谱方法探讨了以对称型pF3N子氨基酸卟啉：11R3S0-四-[4-（Boc-苏氨酸）氨基苯基]卟啉（TAPP—Thr—Boc）作为光谱探针测定三一色氨酸的最佳条件．实验结果表明：双-2-乙基己基硫代琥珀酸钠（AOT）表面活性剂的加入能显著增加体系的灵敏度．在最佳实验条件下，体系荧光强度的下降程度与L-氨酸含量在0．10～8．80μg／mL范围内呈线性关系，检出限为0．034μg/mL方法抗干扰能力强，有较好的选择性和灵敏度，用于实际样品仔猪饲料的测定，结果满意．The optimum experimental conditions for the determination of L- tryptophan were investigated by fluorescence spectrophotometry using tetra-[ （4-Bocthreonine）-aminophenylI porphyrin as the fluorescence spectral probe. Experiments indicated that the sensitivity of the system can be enhanced significantly by the addition of bis（2-ethylhexyl）sulfosuecinate sodium salt （AOT）. Under optimum experimental conditions, the decreased of the fluorescence intensity of the system was in proportion to the concentration of L-tryptophan in the concentration range of 0.10 - 8.80 μg/mL with a limit of detection of 0. 034 μg/mL. The method has been used in the determination of samples with satisfactory results.

关 键 词：氨基酸卟啉 L-色氨酸 荧光光谱法 荧光探针 

分 类 号：O657.3[理学—分析化学]