RSV毛细支气管炎的肺表面活性蛋白D多态性与TaqMan MGB探针  

The genetic polymorphisms of surfactant protein D in RSV bronchiolitis and the TaqMan MGB probe detect

作　　者：朱亚非[1,2] 林艳[1,2] 王晓芳[1,2] 王晓安[1,2] 肖云斌[1,2] 王琦婧[1,2] 

机构地区：[1]杭州师范大学附属医院 [2]杭州市第二人民医院儿科,浙江杭州310015

出　　处：《中国微生态学杂志》2013年第12期1412-1414,1418,共4页Chinese Journal of Microecology

基　　金：浙江省卫生厅医药卫生科技计划项目(2012KYA156);杭州市科技计划引导项目(2012年)

摘　　要：目的探讨TaqMan MGB探针实时PCR技术检测肺表面活性蛋白D（surfactant protein，SP）-D基冈变异是否与呼吸道合胞病毒（respiratol Tsyneytial virus，RSV）毛细支气管炎易感性有关。方法运用Taq—ManMGB探针检测150例RSV毛细支气管炎和150例健康对照组SP—D基因多态性，并进行基因型、等位基因频率分析。各组的基因型、等位基因频率比较采用χ2检验。结果病例组SP—D Met11 Thr位点TT、CT、CC三种基因型频率分别为7．3％、44．0％、48．7％，T、C等位基因频率分别为29．3％、70．7％。三种基因型及等位基因频率与对照组比较差异有统计学意义（χ2=6．751、5．823，P〈0．05）。结论杭州地区汉族儿童存在SP—D基因多态性，SP—DMet11 Thr位点与RSV毛细支气管炎疾病易感性存在关联，C等位基因可能是RSV毛细支气管炎的易感基因。TaqMan MGB探针实时PCR技术是基因诊断良好技术平台。Objective To develop a TaqMan MGB probe real-time PCR detecting the genetic polymorphisms of surfactant protein D （SP-D） and investigate the association between polymorphisms and RSV in Han racial hangzhou. Methods Genotyping of SP-D polymorphisms was performed by polymerase chain reaction and TaqMan MGB probe in 150 cases of RSV bronchitis （case group） and 150 healthy children （control group） , and the genotype frequencies and allele frequencies were analyzed simultaneously. Each group genotype and allele frequencies were compared withχ2 test. Results The frequencies of TT, TC and CC of SP-D Met11Thr were 7.3% , 44. 0% and 48.7%, respectively, and the allele frequencies of T and C were 29.3% and 70. 7%, respectively. SP-D MetllThr displayed significant association with RSV as compared with control group （χ2 = 6. 751 , 5. 823, P 〈 0. 05）. Conclusion SP-D Metl l Thr gene polymorphisms are both present in Han children in Hangzhou area, Polymorphisms of SP-D Metl 1Thr display significant association with RSV as compared with control group. TaqMan MGB probe real-time PCR platform is a good genetic diagnosis technology.

关 键 词：呼吸道合胞病毒 肺表面活性蛋白 基因多态性 实时PCR 

分 类 号：R392-33[医药卫生—免疫学]