TGF-β_1、EGF在人胎儿晶状体上皮细胞的表达  

THE EXPRESSION OF TGF-β_1 EGF IN EPITHELIAL CELLS OF HUMAN EMBRYO

作　　者：牛嗣云[1] 高福禄[1] 潘林洁[1] 王春燕[1] 王玉平 庞小静[1] 

机构地区：[1]承德医学院基础部,河北承德067000 [2]承德市计划生育服务站

出　　处：《承德医学院学报》2000年第3期11-14,共4页Journal of Chengde Medical University

摘　　要：目的 :观察转化生长因子 β1(TGF- β1)、表皮生长因子 (EGF)在人胎儿晶状体上皮细胞的表达。方法 :用 SP免疫细胞化学法对 48例人胎儿不同胎龄晶状体上皮细胞进行 TGF- β1、EGF免疫细胞化学染色。结果 :TGF- β1、EGF在人胎儿不同胎龄晶状体上皮细胞均有表达。TGF-β1、EGF阳性细胞浆为桔黄色或棕黄色的颗粒状或斑块状 ;并且阳性细胞随着胎龄的增加而减少。经相关分析 :TGF- β1与 EGF呈正相关。结论 :TGF- β1、EGF在人胎儿不同胎龄晶状体上皮细胞均有表达 ,并且随着胎龄的增加而减少 ;TGF- β1与 EGF具有协同作用 。Objective:To observe the expression of TGF β 1 and EGF in lens epithelial cells (LECs)of human embryo.Methods:SP techniques were applied for immunocytochemical staining of LECs of 48 human embryos with TGF β 1 and EGF antibodies.Results:The expression of TGF β 1 and EGF in LECs of different fetal ages were found in this experiment.The positive staining is orange or brown,and was scattered or mottled within the cytoplasm;the number of positive cells decreased with the increase of the felal age.The unanimous correlation had been found between TGF β 1 and EGF.Conclusion:There were all expression of TGF β 1 and EGF in LECs of different fetal ages,and the number of positive cells decreased with the increase of the fetal age;TGF β 1 and EGF have associated action,and stimulate the proliferation of LECs of human embryo.

关 键 词：TGF-Β EGF 胎儿 晶状体上皮细胞 表达 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]