复方中药“禽康散”对人工感染新城疫病毒肉鸡血清免疫球蛋白及新城疫抗体效价的影响  

Changes of blood immune globulin and ND-HI immune globulin by complex Chinese crude drug“Qinkangsan”in chicken artificially challenged by NDV

作　　者：邓惠丹[1] 杨军[1,2] 邓俊良[1] 申渝波[1,3] 左之才[1] 王娅[1] 任志华[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室环境公害与动物疾病四川省重点实验室,四川雅安625024 [2]贵州省毕节市农牧局,贵州毕节551700 [3]通威集团药物研究所,四川成都620000

出　　处：《中国兽医学报》2014年第1期144-147,共4页Chinese Journal of Veterinary Science

基　　金：四川省教育厅自然科学重点项目(2006A009);四川省科技厅应用基础研究项目(2008JY0098);“长江学者和创新团队发展计划”创新团队项目(IRT0848)

摘　　要：10日龄的健康艾维茵肉仔鸡250只，随机分为5组。21日龄时除阴性对照组（Ⅳ组）外均用新城疫病毒（Newcastle disease，virus，NDV）强毒株攻毒，各治疗组（Ⅰ～Ⅲ组）在22～25日龄饲料中分别添加0．5％、1.0％、2．0％的复方中药“禽康散”，第Ⅳ、Ⅴ组（阳性对照组）不用药。在攻毒前1d和攻毒后1、3（用药后2d）、7（停药后2d）、15（停药后10d）d观察血清免疫球蛋白及新城疫抗体效价的变化。结果显示：Ⅴ、Ⅱ组血清IgG在攻毒15d（停药10d）时与Ⅲ、Ⅳ、V组差异极显著（P〈O．01）；Ⅰ～Ⅲ组血清IgA浓度在攻毒后3、7d均明显高于第V组（P〈0．05或0．01），以1％治疗组效果最佳；各组之间血清中IgM含量无显著差异（P〉0．05）。Ⅴ～Ⅲ组新城疫抗体效价在攻毒7d后各阶段均高于阳性对照组（P〈0．05或0．01），以第Ⅲ组最佳。结果表明，日粮添加1．0％～2．0％质量分数的“禽康散”能明显增加血清中IgG、IgA含量和新城疫抗体效价，从而提高机体体液免疫功能，达到提高机体抗NDV的能力。Two hundred and fifty broilers of 10-day-old were divided randomly into 5 groups treated with virus and 0.5 % （ I ）, 1.0 % （ H ）, 2.0 % （ III ）, negative control group （ IV ） and positive control group（V ）. All groups except IV group were inoculated NDV-F48E9 at 21-day-old, then treatment groups were given drug（Qinkangsan） at 0.5%,1.0% and 2.0% in the forage at 22 to 25-day-old. Blood immune globulin and ND-HI immune globulin of broilers were observed at 1 d before and 1,3,7 and 15 d after inoculation. Test resuh.IgG of I , II group was remarkably higher than that of III ,IV and Vgroup at 15 d after inoculation（P〈0.01）. IgA ofI , II and III group was higher than that of V group at 3,7 d after inoculation（P〈0.05 or 0.01） ,IgA of II group was the highes. IgM of all groups were no remarkable difference. ND-HI immune globulin of I , II and III group was higher than that of V group, I; group was the highest. The result indicated that 1.0% and 2.0% complex Chinese crude drug ＂Qinkangsan＂ in the forage could raise humor immune function by increase of IgG,IgA and ND-HI immune globulin, thus build up broilers ＇s resistance to NDV.

关 键 词：复方中药 “禽康散” 新城疫病毒 免疫球蛋白 抗体效价 

分 类 号：S852.65[农业科学—基础兽医学] S859[农业科学—兽医学]