盐析法制备小檗碱壳聚糖纳米载药微球  

Preparation of Berberine Loaded Chitosan Nanoparticles by Salt Precipitation

作　　者：曹力凡 周纲[1] 杨建设[1,2] 俞诗源[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]中国科学院兰州化学物理研究所,兰州730000

出　　处：《中国实验方剂学杂志》2014年第1期5-8,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中科院"西部之光"人才计划项目(20090010);甘肃省自然科学基金青年项目(20100012)

摘　　要：目的：探索制备小檗碱壳聚糖载药纳米微球颗粒的新方法及关键技术。方法：采用盐析法，通过三聚磷酸钠交联制备小檗碱壳聚糖纳米载药微球，测定载药微球的包封率，观察其扫描电镜图及红外光谱。结果：小檗碱颗粒粒径分布于400-500nm，经壳聚糖包裹的小檗碱纳米颗粒形状规则，具有核一壳结构，平均粒径约500mm，包封率约70％。结论：壳聚糖包裹的小檗碱颗粒具有均一的纳米粒径和良好的包封率，为小檗碱缓释用药和靶向给药设计提供参考。Objective： To explore new methodsand key technologies for preparation of berberine drugloaded chitosan microspheres nanoparticles. Method： Salt precipitation was adopted, berberine loaded chitosan nanoparticles were prepared by sodiumtripolyphosphate crosslinking method, then encapsulation efficiency of drugloaded microspheres was measured, load capability of nanopaticles was measured by ultraviolet-visible spectrum and infrared spectrum, while its morphology was tested by scanning electron microscope and particle-size analyzer. Result ： Particle size distribution of berberine particles was 400-500 nm, the average diameter of berberine loaded chitosan nanopartieles was about 500nm with core-shell structure in regular shape, encapsulation efficiency was about 70%. Conclusion： Berberine loaded chitosan nanoparticles had a uniform particle size and good encapsulation effciency, this study could provide a reference for release medication and targeted drug design of berberine.

关 键 词：盐析法 小檗碱 壳聚糖 纳米微球 扫描电镜图 红外光谱 粒径分布 

分 类 号：R944.2[医药卫生—药剂学] R283.6[医药卫生—药学]