毛鸡骨草药材的HPLC指纹图谱  

HPLC Fingerprint of Abrus mollis

作　　者：黄平[1] 李文静[1] 何碧婷 

机构地区：[1]广西中医药研究院,广西中药标准研究重点实验室,南宁530022

出　　处：《中国实验方剂学杂志》2014年第1期66-69,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划项目(桂科能05112001-3A5)

摘　　要：目的:建立毛鸡骨草药材的指纹图谱,为控制和评价药材质量提供依据。方法:用高效液相色谱法,Lichrospher C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%三氟乙酸(35∶65),流速0.8 mL·min-1,检测波长272 nm,柱温室温。测定10批毛鸡骨草药材的指纹图谱,并进行相似度比较。结果:建立了毛鸡骨草药材的HPLC指纹图谱,确定9个共有峰,利用对照品指认其中4个峰。10批毛鸡骨草药材的相似度>0.98。结论:建立的方法快速、准确,为评价毛鸡骨草药材的质量提供参考。Objective： To establish a HPLC fingerprint analysis method for the quality control of Abrus mollis. Method： A HPLC method was adopted. Chromatographic assay was performed on a Lichrospher Clscolumn （4.6 mm × 250 mm, 5 μm） at room temperature with methanol-0. 1% TFA （35 ： 65） as the mobile phase. The flow rate was 0. 8 mL -min^-1 and the detection wavelength was set at 272 nm. 10 batches samples of A. mollis were determined for similarity comparison. Result： A HPLC fingerprint analysis method of A. mollis was established. 9 common peaks were assigned, and 4 peaks of them were confirmed by comparing with reference standards. Similarity of 10 batches of A. mollis was more than 0.98. Conclusion： This method was rapid, accurate and reproducible, it could provide a reference for quality evaluation and control of A. mollis.

关 键 词：毛鸡骨草 质量控制 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]